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oai:riuma.uma.es:10630/404602026-02-03T11:25:39Zcom_10630_2254col_10630_37953

Magnetic Nanoparticles with On-Site Azide and Alkyne Functionalized Polymer Coating in a Single Step through a Solvothermal Process Mora-Cabello, Romualdo Fuentes-Ríos, David Gago, Lidia Cabeza, Laura Moscoso, Ana Melguizo, Consolación Prados, José Sarabia-García, Francisco Ramón López-Romero, Juan Manuel Nanopartículas Polímeros Oxidos de hierro Magnetic Fe3O4 nanoparticles (MNPs) are becoming more im- portant every day. We prepared MNPs in a simple one-step reaction by following the solvothermal method, assisted by azide and alkyne functionalized polyethylene glycol (PEG400) polymers, as well as by PEG6000 and the polyol β-cyclodextrin (βCD), which played a crucial role as electrostatic stabilizers, providing polymeric/polyol coatings around the magnetic cores. Methods: The composi- tion, morphology, and magnetic properties of the nanospheres were analyzed using Transmission Electron and Atomic Force Microscopies (TEM, AFM), Nuclear Magnetic Resonance (NMR), X-ray Diffraction Diffractometry (XRD), Fourier-Transform Infrared Spectroscopy (FT-IR), Matrix-Assisted Laser Desorption/Ionization (MALDI) and Vibrating Sample Magnetometry (VSM). Results: The obtained nanoparticles (@Fe3O4-PEGs and @Fe3O4-βCD) showed diameters between 90 and 250 nm, depending on the polymer used and the Fe3O4·6H2O precursor concentration, typically, 0.13 M at 200 ◦C and 24 h of reaction. MNPs exhibited superparamagnetism with high saturation mass magnetization at room temperature, reaching values of 59.9 emu/g (@Fe3O4-PEG6000), and no ferro- magnetism. Likewise, they showed temperature elevation after applying an alternating magnetic field (AMF), obtaining Specific Absorption Rate (SAR) values of up to 51.87 ±2.23 W/g for @Fe3O4- PEG6000. Additionally, the formed systems are susceptible to click chemistry, as was demonstrated in the case of the cannabidiol-propargyl derivative (CBD-Pro), which was synthesized and covalently attached to the azide functionalized surface of @Fe3O4-PEG400-N3. Prepared MNPs are highly dispersible in water, PBS, and citrate buffer, remaining in suspension for over 2 weeks, and non-toxic in the T84 human colon cancer cell line, Conclusions: indicating that they are ideal candidates for biomedical applications. 2025-10-24T12:54:45Z 2025-10-24T12:54:45Z 2025-10-24T12:54:45Z 2024 journal article Mora-Cabello, R.; Fuentes-Ríos, D.; Gago, L.; Cabeza, L.; Moscoso, A.; Melguizo, C.; Prados, J.; Sarabia, F.; López-Romero, J.M. Magnetic Nanoparticles with On-Site Azide and Alkyne Functionalized Polymer Coating in a Single Step through a Solvothermal Process. Pharmaceutics 2024, 16, 1226. https://doi.org/10.3390/pharmaceutics16091226 https://hdl.handle.net/10630/40460 10.3390/pharmaceutics16091226 eng open access MDPI