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oai:riuma.uma.es:10630/404622026-02-03T11:10:05Zcom_10630_2254col_10630_37953

Psychrobacillus vulpis sp. nov., a new species isolated from faeces of a red fox in Spain. Rodríguez-González, Miguel Ángel Reina, José Carlos Béjar, Victoria Llamas, Inmaculada Baciláceas Genomas Quimiotaxonomía Filogenia Zorro rojo - Enfermedades bacterianas https://openpolicyfinder.jisc.ac.uk/id/publication/14638 A facultative anaerobic, chemoheterotrophic, endospore-forming, Gram-stain-positive rod, designated as strain Z8T , was isolated from red fox (Vulpes vulpes) faeces sampled at Tablas de Daimiel National Park, Ciudad Real, Spain. Strain Z8T grew at 0–37°C (optimum, 28°C), in the presence of 0–5.5% (w/v) NaCl (2.5%, w/v) and at pH 6–10 (pH 7). The strain was motile and positive for catalase, oxidase, H2 S and siderophore production, acid and alkaline phosphatases, and N-acetylglucosamine, adipic acid and malate assimilation. It hydrolysed starch, DNA, l-tyrosine, Tween 20, Tween 80 and lecithovitellin. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Z8T is a member of the genus Psychrobacillus, showing high sequence similarity to Psychrobacillus lasiicapitis NEAU-3TGS17T (99.2%) and Psychrobacillus soli NHI-2TT (99.1%), and around 98% to other known species of the genus Psychrobacillus. Digital DNA–DNA hybridization and average nucleotide identity values were lower than 24 and 79%, respectively, with the most related species. In silico G+C content was 35.9mol%. The major cellular fatty acids of strain Z8T were iso-C14:0 , iso-C15:0 and anteiso-C15:0 . The novel strain contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as predominant polar lipids, and the main respiratory isoprenoid quinone was MK-8. Based on the 16S rRNA phylogenetic analysis, together with MLSA (recA, rpoB and gyrB), phylogenomic, chemotaxonomic and phenotypic results, we demonstrate that strain Z8T represents a novel species of the genus Psychrobacillus, for which the name Psychrobacillus vulpis sp. nov., is proposed. The type strain is Z8T (=CECT 9721T =LMG 31001T ). 2025-10-27T10:17:32Z 2025-10-27T10:17:32Z 2019-12-13 journal article Rodríguez et al., Int. J. Syst. Evol. Microbiol. 2020;70:882–888 DOI 10.1099/ijsem.0.003840 https://hdl.handle.net/10630/40462 10.1099/ijsem.0.003840 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Miicrobiology Society