2026-05-28T07:40:10Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/450202026-01-30T00:46:42Zcom_10630_2254col_10630_37953

Borroto Escuela, Dasiel Óscar Narváez-Peláez, Manuel Marcellino, Daniel Parrado-Romero, Concepción Narváez-Bueno, José Ángel Tarakanov, Alexander O. Agnati, Luigi F. Díaz-Cabiale, Zaida Fuxe, Kjell Díaz-Cabiale, Zaida 2026-01-29T11:00:41Z 2010-03-19 Dasiel O. Borroto-Escuela, Manuel Narvaez, Daniel Marcellino, Concepción Parrado, José Angel Narvaez, Alexander O. Tarakanov, Luigi F. Agnati, Zaida Díaz-Cabiale, Kjell Fuxe, Galanin receptor-1 modulates 5-hydroxtryptamine-1A signaling via heterodimerization, Biochemical and Biophysical Research Communications, Volume 393, Issue 4, 2010, Pages 767-772, ISSN 0006-291X, https://doi.org/10.1016/j.bbrc.2010.02.078. (https://www.sciencedirect.com/science/article/pii/S0006291X10002986) 10902104 https://openarchive.ki.se/articles/journal_contribution/Galanin_receptor-1_modulates_5-hydroxtryptamine-1A_signaling_via_heterodimerization/27134091. https://doi.org/10.5281/zenodo.13858593. https://hdl.handle.net/10630/45020 10.1016/j.bbrc.2010.02.078 Previous biochemical, cardiovascular and behavioral work has given evidence for the existence of antagonistic galanin receptor-5-HT1A receptor interactions in the brain. In this study we investigated the existence of GalR1-5-HT1A receptor heteromers and their functional characteristics. In mammalian cells transfected with GFP2-tagged 5-HT1A receptor and YFP-tagged GalR1 receptor, a proximity-based fluorescence resonance energy transfer technique was used and it has been demonstrated that GalR1-5-HT1A receptors heteromerize. Furthermore, signaling by either the mitogen-activated protein kinase (MAPK) or adenylyl cyclase (AC) pathways by these heteromers indicates a trans-inhibition phenomenon through their interacting interface via allosteric mechanisms that block the development of an excessive activation of Gi/o and an exaggerated inhibition of AC or stimulation of MAPK activity. The presence of these heteromers in the discrete brain regions is postulated based on the existence of GalR-5-HT1A receptor-receptor interactions previously described in the brain and gives rise to explore possible novel therapeutic strategies for treatment of depression by targeting the GalR1-5-HT1A heteromers. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 International Neurorreceptores Neuropéptidos Galanin receptor-1 modulates 5-hydroxtryptamine-1A signaling via heterodimerization journal article