2026-06-04T04:41:50Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/451412026-02-04T00:47:21Zcom_10630_2254col_10630_37953

00925njm 22002777a 4500 dc Carmona Martín, Elisabeth author Regalado, José Javier author Perán-Quesada, Rosa Amelia author López Encina, Carlos author 2018-02-09 We describe an encapsulation–dehydration procedure with prefreezing steps for the cryopreservation of rhizome bud explants of Asparagus officinalis L. cv. Morado de Huétor. With this procedure, survival of Rhizome buds was at least 84 and 42% developed to complete plantlets at 8 weeks. Flow cytometry and EST-SSR molecular markers were used to assess genetic stability of the regenerated material. Effects of preculture time in a medium rich in sucrose and prefreezing treatments (0 °C or/and − 20 °C) on plant recovery were evaluated. Rhizome Buds of the “Morado de Huétor” landrace were incubated in preculture medium (MS + 0.3 M sucrose) for 48 h, encapsulated in alginate beads and desiccated until a water content of 35%, prefrozen for one hour at 0 °C plus one hour at − 20 °C, followed by cryopreservation in liquid nitrogen, and then were rewarmed and recovered in ARBM medium for 6 weeks and finally incubated in ARBM-0 for 4 weeks. Analyses of ploidy and molecular stability of plantlets recovered from cryopreserved rhizome buds of two selected genotypes showed no differences compared with the mother plants. Cryopreservation of RB explants of A. officinalis with this Encapsulation–Dehydration procedure will be useful in long-term preservation programs. Carmona-Martín, E., Regalado, J.J., Perán-Quesada, R. et al. Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability. Plant Cell Tiss Organ Cult 133, 395–403 (2018). https://doi.org/10.1007/s11240-018-1392-y 1573-5044 https://hdl.handle.net/10630/45141 10.1007/s11240-018-1392-y Espárragos Crioconservación Marcadores biológicos Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability