2026-05-28T00:31:12Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/452552026-02-07T00:46:03Zcom_10630_2254col_10630_37959

00925njm 22002777a 4500 dc Castro-Rodríguez, Vanessa author Floor, Martin author Robles, Ana author Ávila-Sáez, Concepción author Cánovas-Ramos, Francisco Miguel author 2025 Nitrogen (N) is a key player of plant growth and development, yet its excessive use in fertilizers leads to severe environmental consequences, including groundwater contamination and ecosystem disruption. Understanding nitrogen assimilation, transport, and biosynthesis at the cellular and subcellular levels is essential for optimizing plant productivity and sustainability. However, a major challenge persists: the lack of tools for real-time monitoring of nitrogen-related processes in living plants. To bridge this gap, we propose the development of dual ratiometric biosensors equipped with fluorescent proteins, designed to track nitrogen transport dynamics across different cellular compartments. Inspired by recent advances in biosensors in plants, our approach leverages key nitrogen transporters such as the ammonium transporter PpAMT1.3 from Pinus pinaster. By integrating these sensors, we aim to unveil the spatial distribution and functional mechanisms of nitrogen transporters at a level of resolution never before achieved. Studying ammonium transport in conifers is particularly relevant due to their dominance in temperate and boreal forests, where nitrogen availability is a limiting factor for growth. Unlike agricultural crops, conifers rely on highly efficient nitrogen uptake and recycling mechanisms to grow in nutrient-poor soils. Understanding how ammonium is transported and assimilated in these trees could provide critical insights for improving forest resilience, carbon sequestration, and sustainable management strategies in the face of climate change. This innovative strategy holds the potential to revolutionize plant nitrogen research, showing the way for more efficient nutrient use and sustainable agricultural practices, while offering valuable insights into plant physiology and real-time monitoring of nutrient dynamics, thus enhancing our understanding of plant responses and enabling more informed decision-making in agricultural management.‍ https://hdl.handle.net/10630/45255 Biosensores Plantas Nitrógeno Illuminating nitrogen transport: engineering biosensors for real-time monitoring in plants