2026-06-06T00:50:01Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/454172026-04-10T09:19:47Zcom_10630_2254col_10630_37953

Dendrimeric antigens for passive mast cell activation in the evaluation of amoxicillin allergy Tesfaye Ayane, Amene Salas, María Benede, Sara Rodríguez-Sánchez, Maria J. Rodríguez-Sojo, María J. Jiménez-Sánchez, Isabel M. Bogas, Gador Pérez-de-Inestrosa-Villatoro, Ezequiel Mayorga, Cristobalina Paris, Juan L. Rodríguez-Nogales, Alba Torres-Jaén, María Josefa Montañez-Vega, María Isabel Antibióticos Alergia a los medicamentos Antibióticos betalactámicos Amoxicilina Background: Amoxicillin (AX) is frequently implicated in immediate IgE-mediated allergic reactions. Diagnosis is challenging,highlighting the need for new approaches enhancing in vitro sensitivity and specificity. Engineered nanostructures can mimicimmunological recognition of hapten-carrier conjugates, offering a strategy to improve diagnostic accuracy.Methods: Dendrimeric Antigens (DeAns), with controlled size (1st–5th generation) and multivalent AX determinants (8–128 units, respectively), were synthesized for in vitro immunological evaluation. In vitro IgE recognition was studied by com-petitive radio-immunoassay. Allergenic activity was evaluated in mouse bone marrow-derived mast cells (MC) sensitized withmouse anti-AX IgE monoclonal antibody and humanized RBL-2H3 (huRBL-2H3) and LUVA cells sensitized with sera fromβ-lactam-allergic subjects and tolerant controls, measuring degranulation in response to DeAns stimulation.Results: Five different DeAns were obtained as pure compounds. All DeAns were recognized by AX-sIgE. A clear size-dependent activation pattern was observed in the three cell models: lower-generation (1st–2nd) DeAns failed to induce degranu-lation, whereas DeAns of bigger size (3rd–5th generation) triggered significant, dose-dependent activation. Notably, no activationwas observed in tolerants and unsensitized cells or with blank dendrimers. In patient-sera assays, the passive MC activation test(pMAT) with DeAns provided complete diagnostic discrimination, with activation restricted to AX-allergic patients.Conclusions: DeAns are effective platforms for investigating effector cell activation in AX allergy. By fine-tuning structuralattributes—size and multivalence—we reveal the promising utility of DeAns in pMAT that leverage commercial cell lines andpatient sera. This approach could address key limitations of β-lactam allergy diagnostics, enabling more reliable and standard-ized in vitro testing. 2026 journal article A. T. Ayane, M. Salas, S. Benede, et al., “ Dendrimeric Antigens for Passive Mast Cell Activation in the Evaluation of Amoxicillin Allergy,” Allergy (2026): 1–12, https://doi.org/10.1111/all.70250. https://hdl.handle.net/10630/45417 https://doi.org/10.1111/all.70250 eng info:eu-repo/grantAgreement/EC/H2020/713721/EU info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII)/AC19%2F00082/ES/NANODIAGNOSTICO PARA LA HIPERSENSIBILIDAD A BETALACTAMICOS/ info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 (ISCIII)/PI17%2F01237/ES/APLICACION DE NANOARQUITECTURAS SINTETICAS AL ESTUDIO DE LOS MECANISMOS DE ACTIVACION DEL BASOFILO Y AL DIAGNOSTICO EN REACCIONES ALERGICAS A AMOXICILINA./ info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII)/PI21%2F00329/ES/OPTIMIZACION DIAGNOSTICA EN ALERGIA A BETALACTAMICOS: DE LA ESTANDARIZACIÓN Y RACIONALIZACIÓN DE LAS PRUEBAS IN VIVO, AL DESARROLLO DE TEST IN VITRO INNOVADORES BASADOS EN NANOTECNOLOGIA Y METABOLÓMICA./ info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII)/PI20%2F01734/ES/NANOARQUITECTURAS ALTAMENTE EFICIENTES PARA UN ENTRECRUZAMIENTO EFICAZ DE LA IGE EN MASTOCITOS Y BASOFILOS. APLICACION AL DIAGNOSTICO DE REACCIONES ALERGICAS A AMOXICILINA Y ACIDO CLAVULANICO./ http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 International Wiley