2026-06-01T11:58:01Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/454192026-02-13T00:45:17Zcom_10630_2254col_10630_37953
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Palmquist-Gomes, Paul
author
Pérez-Pomares, José María
author
Guadix-Domínguez, Juan Antonio
2026-02-12T12:31:30Z
2019-02
Palmquist-Gomes P, Pérez-Pomares JM, Guadix JA. Proepicardial Origin of Developing Coronary Vessels. Rev Esp Cardiol (Engl Ed). 2019 Feb;72(2):163. English, Spanish. doi: 10.1016/j.rec.2018.01.028. Epub 2018 May 4. PMID: 29735303.
https://hdl.handle.net/10630/45419
10.1016/j.rec.2018.01.028
Coronary irrigation of the myocardium is essential for homeostasis in the adult heart, and embryonic developmental abnormalities in these blood vessels may result in congenital heart defects. Despite the clinical relevance of coronary arteries, the embryonic mechanisms regulating their formation are not known in detail. Recent studies suggest that the epicardial progenitors (proepicardial cells [EP]) make a critical contribution to coronary artery morphogenesis. Transplantation of proepicardial quail cells to chicken hearts (Figure A) can be used to trace the destination of the donor cells in chimeric embryos, as endothelial quail cells–but not chicken cells–are labeled with QH1 antibody (Figure B-E, red signal; Ao, aorta; LA, left arium; LV, left ventricle; PA, pulmonary artery; RA, right atrium; RV, right ventricle). Intravascular injection of fluorescein-conjugated Lens culinaris lectin (FITC-LC) labels all blood vessels connected with systemic circulation (Figure B-E, green signal). The ventral views (Figure B) and dorsal views (Figure D; VS, venous sinus) of a chimeric heart (7 days of incubation) show how donor EP-derived endothelial cells (QH1+) form coronary vessels in heart chambers but not at the arterial pole of the heart (Figure B and C, discontinuous line), and become incorporated into the endothelium of coronary vessels whether connected (FITC-LC+, arrowheads of Figure C and E) or not connected with systemic circulation (FITC-LC–) (Figure C and E, arrows). The FITC-LC+/QH1– vessels are not derived from the quail EP.
eng
Miocardio
Endotelio
Proepicardial Origin of Developing Coronary Vessels
journal article
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