2026-05-27T05:29:41Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/455642026-02-20T00:46:14Zcom_10630_2254col_10630_37953

Live cell imaging reveals 3′-UTR dependent mRNA sorting to synapses Bauer, Karl E. Segura, Inmaculada Gaspar, Imre Scheuss, Volker Illig, Christin Ammer, Georg Hutten, Saskia Basyuk, Eugénia Fernández-Moya, Sandra M. Ehses, Janina Bertrand, Edouard Kiebler, Michael A. Ácido ribonucleico mensajero mRNA transport restricts translation to specific subcellular locations, which is the basis for many cellular functions. However, the precise process of mRNA sorting to synapses in neurons remains elusive. Here we use Rgs4 mRNA to investigate 3′-UTR-dependent trans- port by MS2 live-cell imaging. The majority of observed RNA granules display 3′-UTR independent bidirectional transport in dendrites. Importantly, the Rgs4 3′-UTR causes an anterograde transport bias, which requires the Staufen2 protein. Moreover, the 3′-UTR mediates dynamic, sustained mRNA recruitment to synapses. Visualization at high temporal resolution enables us to show mRNA patrolling dendrites, allowing transient interaction with multiple synapses, in agreement with the sushi-belt model. Modulation of neuronal activity by either chemical silencing or local glutamate uncaging regulates both the 3′-UTR-depen- dent transport bias and synaptic recruitment. This dynamic and reversible mRNA recruitment to active synapses would allow translation and synaptic remodeling in a spatially and tem- porally adaptive manner. 2026-02-19T08:52:09Z 2019-07-18 journal article Bauer, K.E., Segura, I., Gaspar, I. et al. Live cell imaging reveals 3′-UTR dependent mRNA sorting to synapses. Nat Commun 10, 3178 (2019). https://doi.org/10.1038/s41467-019-11123-x https://hdl.handle.net/10630/45564 10.1038/s41467-019-11123-x eng http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 International Springer Nature