2026-06-07T07:14:38Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/458352026-02-28T00:46:47Zcom_10630_2254col_10630_37956

Generating chromosome-located transcriptional fusions to fluorescent proteins for single-cell gene expression analysis in Pseudomonas syringae. Rufián Plaza, José Sebastián López-Márquez, Diego López-Pagán, Nieves Grant, Murray Ruiz-Albert, Javier Beuzón-López, Carmen del Rosario Medina, Carlos López Baena, Francisco Javier Genética bacteriana Fenotipo Microscopía de fluorescencia https://www.springernature.com/gp/open-science/policies/book-policies The last decade has seen significant effort directed toward the role of phenotypic heterogeneity in bacterial adaptation. Phenotypic heterogeneity usually refers to phenotypic diversity that takes place through nongenetic means, independently of environmental induced variation. Recent findings are changing how microbiologists analyze bacterial behavior, with a shift from traditional assays averaging large populations to single-cell analysis focusing on bacterial individual behavior. Fluorescence-based methods are often used to analyze single-cell gene expression by flow cytometry, fluorescence microscopy and/or microfluidics. Moreover, fluorescence reporters can also be used to establish where and when are the genes of interest expressed. In this chapter, we use the model bacterial plant pathogen Pseudomonas syringae to illustrate a method to generate chromosome-located transcriptional gene fusions to fluorescent reporter genes, without affecting the function of the gene of interest. 2026-02-27T14:02:26Z 2018-01-01 book part Rufián, J.S., López-Márquez, D., López-Pagán, N., Grant, M., Ruiz-Albert, J., Beuzón, C.R. (2018). Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae . In: Medina, C., López-Baena, F. (eds) Host-Pathogen Interactions. Methods in Molecular Biology, vol 1734 978-1-4939-7603-4 https://hdl.handle.net/10630/45835 10.1007/978-1-4939-7604-1_15 eng open access Springer Nature