2026-06-01T07:08:14Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/460342026-03-17T09:50:06Zcom_10630_2254col_10630_20092

Dataset Hippocampal proteomics in a mice model of early life adversity in combination with a second acute stress in adulthood. Muñoz-Martín, José Pérez-Martín, Margarita Pedraza-Benítez, María del Carmen Estrés (Fisiología) Hipocampo (Cerebro) Proteómica Psicopatología Trauma psíquico Proteomic analysis was performed on left hippocampal tissue from C57BL/6J mice subjected to maternal separation during infancy (MS), acute restriction stress in adulthood (RS), or combined MS+RS, compared to controls (CTRL). N=5-6 per group, males and females separately. Hippocampi were collected 24h post RS stress (P62-P65), homogenized in RIPA buffer, quantified by BCA, and digested via gel-assisted proteolysis with trypsin. Samples underwent UHPLC-HRMS on a Q-Exactive HF-X , acquiring MS/MS spectra (SCAI-Universidad de Málaga). Raw data were searched in Proteome Discoverer 2.5 against Mus musculus UniProt (2025-02), yielding 2,895 high-confidence proteins (FDR<1%, ≥2 peptides, master proteins). Label-free quantification used precursor intensities, normalized by total peptide amount; ANOVA identified upregulated (p<0.05, log2 FC≥1) and downregulated proteins (p<0.05, log2FC ≤-1) in the following comparisons: RS vs CTRL, MS vs CTRL, MS+RS vs CTRL, RS vs MS+RS, MS vs MS+RS (males and females, separately). Raw proteomic data as well as volcano plots are distributed in RIUMA (Universidad de Málaga). 2026 dataset https://hdl.handle.net/10630/46034 10.24310/riuma.46034 eng PID2020-117464RB-I00 PID2023-151537OB-I00 P20_00460 http://creativecommons.org/licenses/by-nc-sa/4.0/ open access Open Data Commons Attribution License (ODC-By) Attribution-NonCommercial-ShareAlike 4.0 International Universidad de Málaga