2026-06-01T13:30:49Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/56752026-02-03T12:11:09Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Palomo-Ríos, Elena
author
Vidoy-Mercado, Isabel
author
Barceló-Muñoz, Araceli
author
Mercado-Carmona, José Ángel
author
Pliego-Alfaro, Fernando
2013-09-04T06:20:51Z
2013-09-04T06:20:51Z
2013-06-02
8th International Symposium on In vitro culture and Horticultural Breeding, University of Coimbra, Portugal, June 2-7, Libro de Abstracts, pág.53
http://hdl.handle.net/10630/5675
Use of biotechnological tools in avocado (Persea americana Mill.) is hampered by difficulties in obtaining mature somatic embryos with an acceptable germination capacity. Use of semi-permeable cellulose acetate membranes on top of maturation medium has improved the quality of obtained embryos and their germination rate; however, in the case of transgenic embryos the conversion rate is still rather low. In this investigation, a protocol for recovery of transgenic plants has been developed. Mature avocado somatic embryos, over cellulose acetate membranes, were obtained and induced to germinate following the protocol described in Palomo-Ríos (2012). Some transgenic embryos developed buds ≤ 2 mm, which failed to elongate. For shoot recovery, cotyledons were partially removed and the embryonic axis cultured over 4 weeks in MS medium supplemented with either 1 mg/l BA, 1 mg/l TDZ or 1 mg/l BA and 1 mg/l TDZ. Highest shoot recovery was obtained in medium supplemented with 1 mg/l BA and 1 mg/l TDZ, 53.6±6.7% versus 23.2±5,7% and 39.6±6.4% in media supplemented with 1 mg/l BA or 1 mg/l TDZ, respectively. Afterwards, sprouted embryos were cultured over 4 additional weeks in MS medium supplemented with 1 mg/l BA. In some cases, resulting shoots could be induced to proliferate in GD medium (Gamborg et al., 1968) supplemented with 0.3 mg/l BA, while in other cases, they had to be recovered through micrografting onto in vitro germinated seedlings as described in Pliego-Alfaro and Murashige (1987). MS medium supplemented with 1mg/l BA and solidified with 6 g/l Sigma A-1296 agar, was used for micrografts. Shoots derived from micrografts were either induced to proliferate in GD medium with 0.3 mg/l BA or further grafted and cultured in liquid MS medium supplemented with 0.1 mg/l BA, using perlite as substrate. After 8-12 weeks, micrografts could be transferred to ex vitro conditions. References: Gamborg O.L., Muller, R.A., Iojima, K., (1968). Nutrient requirements of suspension cultures of soybean roots cells. Exp. Cell. Res. 50:151-158. Palomo-Ríos, E., (2012). Thesis: Regeneración y Transformación Genética de Aguacate (Persea americana Mill.). Ph.D. Dissertation, University of Málaga, Málaga Pliego-Alfaro, F., Murashige, T., (1987). Possible rejuvenation of adult avocado by graftage onto juvenile root stocks in vitro. Hort Sci. 22:1321-1324.
eng
Plantas transgénicas
Enhancing shoot recovery from transgenic avocado somatic embryos
conference output
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Abstract Portugal.pdf
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Abstract Portugal.pdf.txt