2026-05-27T04:42:44Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/77012026-02-03T11:59:49Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
El-Azaz-Ciudad, Jorge
author
Cánovas-Ramos, Francisco Miguel
author
De-la-Torre-Fazio, Fernando Nicolás
author
Ávila-Sáez, Concepción
2014-06-19T12:45:42Z
2014-06-19T12:45:42Z
2014-06-19
http://hdl.handle.net/10630/7701
In plants, arogenate dehydratase activity (ADT, EC 4.2.1.91) is responsible for
the last step in the main pathway for phenylalanine biosynthesis, known as the
arogenate pathway, which consist in two steps: the conversion of prephenate to
arogenate in a reaction catalyzed by the enzyme prephenate aminotransferase (PAT, EC
2.6.1.78) and the decarboxylation of arogenate to render phenylalanine catalyzed by
ADT. The arogenate pathway results of particular interest according to the important
role of phenylalanine in plant metabolism, acting as the main gate of entry to
phenylpropanoids biosynthesis, that constitute up to 30 to 45% of plant organic matter
(Razal et al., 1996). This is particularly relevant in perennial woody plants, in which
lignification process and resultant biomass acumulation through plant life cycle are
notably important.
Despite of the high importance of phenylalanine biosynthesis and derived
phenylpropanoids in plants biology, the arogenate pathway still remains poorly
characterized, particularly in woody plants. Very recently, two independent publications
reported physiological evidences suggesting an alternative arogenate-independent
pathway for phenylalanine biosynthesis in plants (Yoo et al., 2013; De la Torre et al.,
2014), as described previously in fungi and bacteria. This pathway is dependent of a
prephenate dehydratase enzyme (PDT, EC 4.2.1.51) catalyzing the conversion of
prephenate to phenylpyruvate, being subsequently converted into phenylalanine through
a transamination reaction. It has been reported that ADT and PDT activities are housed
in the same proteins in plants (Cho et al., 2007).
Here we present preliminary results focused on the characterization of the
ADT/PDT gene family in maritime pine (Pinus pinaster Ait.), a conifer tree of
ecological and commercial interest. Our results demonstrate the existence of at least 9
ADT-like genes in the P. pinaster transcriptome, showing organ- and developmentspecific
mRNA and protein expression profiles. Moreover, 3 of those 9 candidate genes
present a distinctive phylogenetic clustering, forming a conifer-characteristic group of
ADT-like genes differenced from the remaining ADT sequences. These findings
highlights the potential importance of ADT/PDT activities in conifer metabolism,
suggesting the existence of a singular and highly-specialized prephenate-related
metabolism in conifers.
Cho MH, Corea OR, Yang H, Bedgar DL, Laskar DD, Anterola AM, Moog-Anterola FA, Hood RL,
Kohalmi SE, Bernards MA, Kang C, Davin LB and Lewis NG. (2007) Phenylalanine biosynthesis in
Arabidopsis thaliana. Identification and characterization of arogenate dehydratases. J Biol Chem.
282(42):30827-35.
eng
Fenilalanina
Phenylalanine biosynthesis: the role and evolution of arogenate dehydratase gene family in c
conference output
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
URL
https://riuma.uma.es/bitstreams/2d1d4a14-b96f-4f42-8db8-6bfecb9010be/download
File
MD5
52d66ce794ed7169565a8dc841c2ec3d
11825
application/pdf
Abstract JORGE CARTAGENA.pdf
URL
https://riuma.uma.es/bitstreams/6f930bbe-da9b-4fab-a033-4c2f24c5edaa/download
File
MD5
a55b9452874a390f295c78b705a000e0
3830
text/plain
Abstract JORGE CARTAGENA.pdf.txt