2026-05-28T19:50:03Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/78572026-02-03T12:14:53Zcom_10630_2254col_10630_37959

00925njm 22002777a 4500 dc Mesa, Pablo author Collado-Martín, Daniel author Vida-Pol, Yolanda author Nájera-Albendín, Francisco author Pérez-de-Inestrosa-Villatoro, Ezequiel author 2014-07-17 We present here the synthesis and characterization of BAPAD [1] fluorescent Dendrimeric-Antigens (DeAn), to be applied in basophil activation as a test to detect drug allergy reactions. A second generation BAPAD dendrimer using cystamine as core that can be reducced to obtain two dendrons with a thiol group in the focal point, useful to attach a fluorescent probe, have been synthesized. The free amine groups on the surface of the dendrimer were functionalized with an amoxiciloyl group (AXO), the allergenic determinant to the beta-lactam antibiotic amoxicillin. The fluorescent DeAn (F-DeAn) has been fully characterized by NMR and MS techniques, and their fluorescent properties well established in aqueous biological media. F-DeAn without the haptenic moieties at the periphery has been also obtained and fully characterized as a control assay. Both molecules have been also characterized using molecular dynamics simulation calculations. We show here how these dendrimeric structures interacts with dendritic cells and are internalized by them. In a secon approximation, Naphthalimide-fluorscent silica nanoparticles have been prepared and characterized, to be used also in basophil activation test. http://hdl.handle.net/10630/7857 Fotoquímica Preparation, photophysical properties and cell image of Naphthalimide-based fluorescent nanostructures for biomedical applications: BAPAD dendrimeric antigens and silica nanoparticles