2026-05-30T05:03:34Zhttps://riuma.uma.es/rest/oai/requestoai:riuma.uma.es:10630/79422026-02-03T11:49:36Zcom_10630_2254col_10630_37959
RIUMA. Repositorio Institucional de la Universidad de Málaga
author
Caro-Astorga, Joaquín
author
Pérez-García, Alejandro
author
Romero, Diego
author
De-Vicente-Moreno, Antonio
2014-07-28T09:40:18Z
2014-07-28T09:40:18Z
2014-07-28
http://hdl.handle.net/10630/7942
One of the greatest challenges of the food industry is to reduce food poisoning caused by food-borne pathogenic bacteria. Bacillus cereus is one the recurrent bacterial pathogens responsible of many outbreaks. Biofilm formation and sporulation are thought as the most important reservoirs of B. cereus. Biofilms are bacterial communities difficult to eradicate from biotic and abiotic surfaces. They are usually composed of exopolysaccharides and proteins. In our study, we are interested in the characterization of the molecular bases leading to biofilm formation in B. cereus during the interaction with plants. To start this analysis we have focused on the structural components of the extracellular matrix of these biofilms. Using as a model the well-studied B. subtilis, we show in this work the presence in B. cereus of an orthologous of tasA. TasA forms amyloid-like fibers in the extracellular matrix of B. subtilis. We demonstrate that the heterologous expression of this allele in a B. subtilis strain lacking the entire tasA operon and unable to form biofilm, restores the assembly of biofilm formation. Biochemical and morphological studies of this protein let us think on a possible amyloid-like nature of this protein, which could be involved in cell-cell interaction and biofilm formation as it does in B. subtilis
eng
Biopelículas
Bacillus cereus
Adhesin proteins in biofilms of the food-borne pathogen Bacillus cereus
conference output
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URL
https://riuma.uma.es/bitstreams/c089a0b1-224a-48a7-9319-6b47e90e59ec/download
File
MD5
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application/pdf
2014.03.13. Abstract.Grecia.pdf
URL
https://riuma.uma.es/bitstreams/d0dcd491-c5ec-4b2e-b92f-a5c12a3d3280/download
File
MD5
9067e31e68eae66f28e97391df3f91df
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text/plain
2014.03.13. Abstract.Grecia.pdf.txt