2026-05-27T05:32:33Zhttps://riuma.uma.es/rest/oai/request

oai:riuma.uma.es:10630/96582026-02-03T12:14:17Zcom_10630_2254col_10630_37959

Van der Selt, Marcelis 2015-04-15T09:58:39Z 2015-04-15T09:58:39Z 2015-04-15 http://hdl.handle.net/10630/9658 Endocannabinoids play an essential role in human health and disease, regulating processes such as immunomodulation, energy balance and neurotransmission. Diacylglycerollipase-α (DAGL-α) is responsible for the production of the endocannabinoid 2-arachidonoylglycerol (2-AG) in the central nervous system. It is a potential drug target for the treatment of obesity and neurodegenerative diseases. Currently, there are no selective inhibitors and activity-based probes available for its study. The identification of selective DAGL- inhibitors is hampered by a lack of assays that make use of endogenous DAGL- activity in proteomes. Determination of the selectivity of the inhibitors in native tissues is important, because DAGL- belongs to the class of serine hydrolases, containing more than 200 members with various physiological functions. Here, I will present a chemical biology approach to identify and characterize highly selective chemical probes to study the function of this protein both in vitro and in vivo. eng open access Receptores de neurotransmisores A chemical biology approach to control endocannabinoid biosynthesis conference output