David Ladrón de Guevara-Miranda, Román Darío Moreno-Fernández, Sara Gil-Rodríguez, Cristina Rosell-Valle, Guillermo Estivill-Torrús, Antonia Serrano, Francisco Javier Pavón, Fernando Rodríguez de Fonseca, Luis Javier Santín, and Estela Castilla-Ortega. Lysophosphatidic acid-induced increase in adult hippocampal neurogenesis facilitates the forgetting of cocaine-contextual memory.

Authors are from  Departamento de Psicobiología y Metodología de las Ciencias del Comportamiento, Instituto de Investigación Biomédica de Málaga (IBIMA), Facultad de Psicología, Universidad de Málaga, Spain; Unidad de Producción de Reprogramación Celular, GMP-Iniciativa Andaluza en Terapia Avanzadas, Junta de Andalucía, Spain; Unidad de Gestión Clínica de Neurociencias, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario de Málaga, Spain; Unidad de Gestión Clínica de Salud Mental, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario de Málaga, Spain.

Author contact: Luis Javier Santín, luis@uma.es; Estela Castilla Ortega, ecastilla@uma.es

Data_LPANeurogenesisCocaine
Excel table file containing raw data from Figures 1-4 and Supplementary table S1, divided in sheets, of the related paper.

Figure 1: Impact of chronic intracerebroventricular (i.c.v.) treatments on conditioned place preference (CPP) retention and extinction. 1b CPP-Score; 1c change (Δ) in CPP-Score; 1d Locomotion (cm); 1e CPP-Score in retention and extinction sessions (blocks; 1-12); 1f change (Δ) in extinction sessions (blocks; 1-12); 1g Elevated Plus Maze (EPM) factor scores (Anxiety factor, Exploration factor and Alertness factor); 1h Open Field (OF) factor scores (Thigmotaxis factor, Exploration factor and Self-care factor); 1j Spontaneous alternation score in the Y-Maze.  
Figure 2: Increases in adult hippocampal neurogenesis induced by chronic lysophosphatidic acid (LPA) treatment. 2b BrdU+ cells/mm2; 2c NeuN+BrdU+ cells (%).
Figure 3: Increased adult hippocampal neurogenesis (AHN) led to the reduction of cocaine (COC)-conditioned place preference (CPP) retention in the lysophosphatidic acid (LPA)-treated group. 3a Adult Hippocampal Neurogenesis (BrdU+ cells/mm2); 3b CPP retention (ΔCPP-Score).
Figure 4: Lysophosphatidic acid (LPA) increased hippocampal cell proliferation. 4c PCNA+ cells/mm2 in the Dentate Gyrus (DG) and the SupraDG and InfraDG layers of the DG in Wild-type mice (WT) and maLPA1-null mice.
		
Supplementary table S1: Individual measures assessed in the EPM and OF tests. Indluces dataset from EPM (time in open arms, locomotion in open arms, open arm entries, open arm latency, rearing, grooming and risk-assessment) and OF (time in centre, locomotion in centre, center entries, locomotion in periphery, rearing, risk-assessmenta and grooming).

Groups were coded as:
SAL-VEH: Saline + vehicle.
COC-VEH: Cocaine + vehicle.
COC-LPA: Cocaine + LPA.
COC-KI16425: Cocaine + Ki16425.