• Arabidopsis Synaptotagmins 1 and 3 are involved in lipid homeostasis at ER-PM contact sites under cold stress 

      Amorim-Silva, Vítor; Esteban, Alicia; Castillo Garriga, Araceli; Rosado, Abel; Vanneste, Steffen; [et al.] (2018-07-12)
      As sessile organisms, plants must cope with abiotic stress such as soil salinity, drought, and extreme temperatures. This stress signal can activate a phospholipase C (PLC), which hydrolyses PIP2 to generate IP3 and ...
    • Autophagy is required for strawberry fruit ripening 

      Sánchez-Sevilla, José F.; Botella-Mesa, Miguel Angel; Valpuesta-Fernandez, Victoriano; Sanchez-Vera, Victoria (Frontiers Media SA, 2021-08-27)
      Autophagy is a catabolic and recycling pathway that maintains cellular homeostasis under normal growth and stress conditions. Two major types of autophagy, microautophagy and macroautophagy, have been described in plants. ...
    • Characterization of AtDGK2 in relation to Contact Sites 

      García-Hernández, Selene; Ruiz-López, Noemi; Botella-Mesa, Miguel Angel (2019-05-17)
      Contact Sites are conserved cellular regions where two membranes of different organelles are very close but not merged. Contact sites between the endoplasmic reticulum (ER) and the plasma membrane (ER-PM CS) play important ...
    • Deciphering Strawberry Ripening by Tissue Specific Gene Regulatory Networks 

      Lucas-Reina, Eva; Trapero-Mozos, Almudena; De Luis Balaguer, María Angels; Martín-Pizarro, Carmen; Ramos-Alvelo, Martín; [et al.] (2018-07-12)
      During ripening, fruits undergo a number of metabolic and physiological changes leading to softening and improvement of characters such as flavor and palatability. Insights into transcriptome changes during strawberry fruit ...
    • Deciphering the role of plant Synaptotagmins in the tolerance to multiple stresses 

      Perez Sancho, Jesica (UMA Editorial, 2017-12-01)
      Las células de todos los organismos están sujetas a estrés, entendiendo éste como condiciones ambientales desfavorables que limitan su normal desarrollo. A diferencia de otros organismos, las plantas no pueden escapar ...
    • FaRIF Transcription Factor Plays a Key Role in the Regulation of Fruit Ripening in the Cultivated Strawberry Fragaria x ananassa 

      Martín-Pizarro, Carmen; Meco, Victoriano; Vallarino, José G.; Pillet, Jeremy; Casañal, Ana; [et al.] (2018-07-12)
      Strawberry is becoming a model for studying the molecular mechanism of ripening in non-climacteric fruits. However, a limited number of transcriptional regulators of this process have been identified so far. In this study, ...
    • Farif transcription factor plays a key role in the regulation of fruit ripening in the cultivated strawberry fragaria × ananassa 

      Martín-Pizarro, Carmen; Meco, Victoriano; Urrutia, María; Vallarino, José G.; Pillet, Jeremy; [et al.] (2021-01-22)
      Strawberry is becoming a model for studying the molecular mechanism of ripening in non-climacteric fruits. However, a limited number of transcriptional regulators of this process have been identified so far. In this study, ...
    • Genetic determinants of Vitamin C content in higher plants 

      Fenech-Torres, Mario; Amorim-Silva, Vitor; Smirnoff, Nicholas; Valpuesta-Fernandez, Victoriano; Botella-Mesa, Miguel Angel (2018-07-18)
      Ascorbic acid (AsA, VitC) is the most abundant water-soluble antioxidant in plants and it plays a plethora of biological roles including resistance to abiotic stress. Hence, including VitC as a trait to improve in breeding ...
    • How are DGK1 and DGK2 involved in membrene contact sites? 

      Garía-Hernández, Selene; Ruiz López, Noemi; Botella-Mesa, Miguel Angel (Reunión Biología de Plantas Hispano Luso, 2021-07-07)
      Eukaryotic cells have regions of interaction between two organelles where some proteins, which act as tether, bring both membranes closer (10-30 nm) without fusion, named membrane contact sites (MCS). Two organelles that ...
    • Lipidome remodeling during cold acclimation is controles by plasma membrane associated synaptotagmins 

      Pérez-Sancho, Jessica; Rosado, Abel; Botella-Mesa, Miguel Angel (2013-11-25)
      The lipidome remodeling during cold acclimation is an important evolutionary milestone to plant adaption to sub-optimal temperature conditions. In Arabidopsis, the phospholipid binding protein Synaptotagmin1 (SYT1) ...
    • NAHG increases transient transformation of arabidopsis leaves by agrobacterium 

      Cana-Quijada, Pepe; Rosas-Díaz, Tábata; Amorim-Silva, Vitor; Botella-Mesa, Miguel Angel; Lozano-Durán, Rosa; [et al.] (2018-07-11)
      Agrobacterium tumefaciens-mediated transient transformation has demonstrated to be an invaluable tool in plant molecular biology studies. However, low efficiency and inconsistency of this method in Arabidopsis has forced ...
    • NRT2.5 a putative sodium dependent high affinity nitrate trasnporter of zostera marina l. 

      Díaz-García, Jordi; Fernández, José A.; Botella-Mesa, Miguel Angel; Rubio-Valverde, Lourdes (2018-07-12)
      Seagrasses are the only group of vascular plants that recolonized the marine environment, possibly the most severe habitat shift ever accomplished by flowering plants. These plants have regained functions enabling them to ...
    • Organisation and control of ascorbate biosynthesis pathway in plants 

      Fenech Torres, Mario (UMA Editorial, 2021-02-03)
      Ascorbate, also known as vitamin C, plays fundamental roles in human health. However, we are unable to synthesise ascorbate due to a series of accumulated mutations in the last enzyme of the pathway. Therefore, fruits and ...
    • Plasma membrane lipid remodeling during cold acclimation is mediated by the ER-PM contact sites-localized synaptotagmins 1 and 3 

      Pérez-Sancho, Jessica; Rosado, Abel; Schapire, Arnaldo; Ruiz-Lopez, Noemi; Osorio-Algar, Sonia; [et al.] (2016-10-28)
      Cold acclimation is the capacity of certain plants to increase their freezing tolerance in response to a period of low non-freezing temperatures. Cold acclimation involves a series of biochemical and physiological adaptations, ...
    • REGULATION OF VITAMIN C BIOSYNTHESIS IN HIGHER PLANTS 

      Fenech Torres, Mario; Amorim-Silva, Vitor; Castillo, Araceli G.; Esteban del Valle, Alicia; Valpuesta-Fernandez, Victoriano; [et al.] (2019-05-15)
      Ascorbate, also known as vitamin C, plays fundamental roles in biotic and abiotic stress resistance in plants. In green tissues, ascorbate is mainly synthesized through the Smirnoff-Wheeler pathway, whose regulation is not ...
    • Regulation of Vitamin C content in higher plants 

      Fenech-Torres, Mario; Amorim-Silva, Vitor; Smirnoff, Nicholas; Botella-Mesa, Miguel Angel (2017-06-23)
      Ascorbic Acid (AsA, vitamin C) has multiple biological roles in plants. Although several pathways for the novo synthesis of AsA have been reported, it is the L-Gal, also known as the Smirnoff-Wheeler (SW), the main pathway ...
    • Role pf TTLs proteins in cellulose biosynthesis 

      García-Moreno, Álvaro; Amorim-Silva, Vitor; Valpuesta-Fernandez, Victoriano; Macho, Alberto; Jaillais, Yvon; [et al.] (2018-07-18)
      Abiotic stress cause detrimental yield losses in crop species. Plants are sessile organisms, so the understanding of the plant cell wall, which is the most important component that constitute the plant biomass of crop ...
    • Scaffold-mediated Assembly of the Brassinosteroid Signaling Components by TTL proteins 

      Amorim-Silva, Vitor; García-Moreno, Álvaro; Lakhssassi, Naoufal; Esteban, Alicia; Rosado, Abel; [et al.] (2017-06-23)
      Brassinosteroids (BRs) is a group of steroidal hormones that play critical roles in multiple aspects of plant growth and development. BR stimulation at the PM receptors initiates a series of phosphorylation events enabling ...
    • SMP-CONTAINING PROTEINS AT MEMBRANE CONTACT SITES: SUBCELLULAR LOCALIZATION AND CHARACTERIZATION. 

      Huércano Rubens, Carolina; Percio Vargas, Francisco; Sánchez-Vera, Victoria; Morello-López, Jorge; Botella-Mesa, Miguel Angel; [et al.] (2021)
      Membrane contact sites (MCS) are microdomains where two membranes of two different organelles are in close apposition, but they do not fuse. MCS are essential for non-vesicular transport of lipids. This lipid transport is ...
    • Study of Transcriptional Regulatory Network Controlling Strawberry Fruit Ripening and Quality 

      Martín-Pizarro, Carmen; Urrutia, María; Meco, Victoriano; Sánchez-Gómez, Carlos; Pillet, Jeremy; [et al.] (2021-01-19)
      Ripening is a critical step for the development of flavor quality in fruits. This character has significantly declined in many fleshy fruits over recent decades. This is particularly significant in strawberry (Fragaria × ...