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A spectroscopic analysis of the interaction between MEGA10 and Concanavalin A
Molina-Bolívar, José Antonio; Carnero-Ruiz, Cristóbal
; Galisteo González, Francisco; Aguilera Garrido, Aixa; Gálvez Ruiz, María José (Elsevier, 2019)
The binding of MEGA10 with Concanavalin A (Con A) at pH 7.4 and pH 5 have been studied using steady-state and time-resolved fluorescence, dynamic light scattering (DLS) and Circular Dichroism(CD). Downward curving Stern-Volmer ... -
Assessing the interaction of Hecamegs with Bovine Serum Albumin and its effect on protein conformation: A spectroscopic study
Hierrezuelo Osorio, Jose Manuel; Nieto-Ortega, Belén; Carnero Ruiz, Cristóbal
(Elsevier, 2014)
Interaction of the nonionic surfactant Hecamegs with the plasma protein Bovine Serum Albumin (BSA), and its effect on protein conformation,has been studied using spectroscopic techniques such as steady-state and time-resolved ... -
La desnaturalización de una proteina globular por surfactantes es monotorizada por espectroscopia Raman quiral
Nieto-Ortega, Belén; Hierrezuelo-Osorio, José Manuel; Carnero-Ruiz, Cristóbal
; López-Navarrete, Juan Teodomiro
; Casado-Cordón, Juan
; Ramírez-Aguilar, Francisco Javier
[et al.] (X Simposio de Investigadores Jóvenes, 2013)
Desnaturalización de proteínas por surfactantes ha recibido una gran atencion los últimos años debido a sus implicaciones en productos farmacéuticos, cosméticos. A lo largo de esta comunicacion se demuestra la capacidad ... -
Effect of NaCl on the Self-Aggregation of n-Octyl â-D-Thioglucopyranoside in Aqueous Medium.
Molina-Bolivar, Jose Antonio; Hierrezuelo-Osorio, José Manuel; Carnero-Ruiz, Cristóbal
(ACS, 2006-06-22)
This report investigates the effect of sodium chloride (NaCl) on the micellization, surface activity, and the evolution in the shape and size of n-octyl â-D-thioglucopyranoside (OTG) aggregates. By using surface ... -
Energetics of clouding and size effects in non-ionic surfactant mixtures: The influence of alkyl chain length and NaCl addition
Molina-Bolivar, Jose Antonio; Hierrezuelo-Osorio, José Manuel; Carnero-Ruiz, Cristóbal
(Elsevier, 2012)
The clouding behavior of two non-ionic mixed surfactant systems (n-dodecyl-b-D-maltoside/n-dodecylhexaethylene-glycol and n-decyl-b-D-maltoside/n-dodecyl-hexaethylene-glycol) was investigated over the entire composition ... -
Maslinic acid conjugate with 7-amino-4-methylcoumarin as probe to monitor the temperature dependent conformational changes of human serum albumin by FRET
Molina-Bolívar, José Antonio; Galisteo González, Francisco; Carnero-Ruiz, Cristóbal
; Medina O´Donnell, Marta; Martinez, Antonio; Parra, Andrés[et al.] (Elsevier, 2019)
methylcoumarin is reported. Itwas found that the coumarin-maslinic derivative (MaCo) forms an excellent fluorescence resonance energy transfer (FRET) pair with the tryptophan (Trp) residue of human serum albumin (HSA). ... -
On the Urea Action Mechanism: A Comparative Study on the Self-Assembly of Two Sugar-Based Surfactants.
Hierrezuelo-Osorio, José Manuel; Molina-Bolivar, Jose Antonio; Carnero-Ruiz, Cristóbal
(ACS, 2009-04-24)
Studies on the effect of urea on micelle formation and structure of n-octyl- -D-thioglucoside (OTG) and N-decanoyl-N-methylglucamide (MEGA-10) were carried out by using the steady-state and time-resolved fluorescence ... -
Physicochemical Studies on the Interaction between N-Decanoyl-N-methylglucamide and Bovine Serum .
Carnero-Ruiz, Cristóbal; Hierrezuelo-Osorio, José Manuel
; Aguiar-García, Juan
; Peula-García, José Manuel
(ACS, 2007-07-13)
The protein-surfactant system constituted by bovine serum albumin (BSA) and N-decanoyl-N-methylglucamide (MEGA-10) has been studied by using surface tension, steady-state fluorescence, and dynamic light scattering measurements. ... -
Raman Optical Activity Monitoring the Interaction of Globular Proteins with Surfactants
Nieto-Ortega, Belén; Hierrezuelo-Osorio, José Manuel; Carnero-Ruiz, Cristóbal
; López-Navarrete, Juan Teodomiro
; Casado-Cordón, Juan
; Ramírez-Aguilar, Francisco Javier
[et al.] (2014-03-31)
Protein denaturation by surfactants is related with topic as pharmaceutics, cosmetics, paints or biotechnology. This phenomenon is highly dependent on the physico-chemical properties of the denaturing agents. In this ... -
Unfolding Pathway of a Globular Protein by Surfactants Monitored with Raman Optical Activity
Nieto-Ortega, Belén; Hierrezuelo-Osorio, José Manuel; Carnero-Ruiz, Cristóbal
; López-Navarrete, Juan Teodomiro
; Casado-Cordón, Juan
; Ramírez-Aguilar, Francisco Javier
[et al.] (ACS Publications, 2014)
Protein denaturation by surfactants has received increased attention in the last years due to its implications in topics such as pharmaceutics, cosmetics, paints, or biotechnology. This phenomenon is highly dependent on ...