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Mostrando ítems 1-15 de 15
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A core collection of modified strawberry germplasm as a resource tool for funghi infection and fruit texture studies
Pineda, Hugo; Pose-Albacete, Sara; Paniagua Correas, Candelas; García-Gago, Juan Antonio; Pliego-Alfaro, Fernando; Quesada-Felice, Miguel Ángel; Mercado-Carmona, José Ángel; Matas-Arroyo, Antonio Javier[et al.] (2013-09-24)Strawberry has been extensively used as a model organism for functional genomics of genes related with fungi infection (Amil-Ruiz et al, 2011) and fruit ripening, specially fruit texture (Posé et al, 2011). These studies ... -
A nanostructural view of the cell wall disassembly process during fruit ripening and postharvest storage by atomic force microscopy
Posé-Albacete, Sara; Paniagua Correas, Candelas; Matas, Antonio J.; Gunning, A. Patrick; Morris, Victor J.; Quesada-Felice, Miguel Ángel; Mercado, Jose A[et al.] (Elsevier, 2018-02-13)Este artículo revisa las principales características del proceso de desensamblaje de la pared celular asociado al reblandecimiento de los frutos desde un punto de vista nanoestructural, según lo proporcionado por estudios ... -
AFM study of strawberry pectin nanostructure and its relevance on fruit texture
Pose-Albacete, Sara; Santiago-Doménech, Nieves; Matas-Arroyo, Antonio Javier; Kirby, Andrew R.; Gunning, A. Patrick; Morris, Victor J.; Quesada-Felice, Miguel Ángel; Mercado-Carmona, José Ángel; Paniagua Correas, Candelas[et al.] (2018-10-17)Atomic force microscopy (AFM) has been used to characterize the nanostructure of cell wall pectins during strawberry fruit growth and ripening, as well as in transgenic fruits with pectinase genes downregulated. This ... -
Antisense down-regulation of the strawberry β-galactosidase gene FaβGal4 increases cell wall galactose levels and reduces fruit softening
Paniagua Correas, Candelas; Blanco-Portales, Rosario; Barceló-Muñoz, Marta; García-Gago, Juan Antonio; Waldron, Keith W.; Quesada-Felice, Miguel Ángel; Muñoz-Blanco, Juan; Mercado, José A.[et al.] (Oxford University Press, 2015-11-19)Este estudio investigó el papel del gen FaβGal4 en el reblandecimiento de la fresa durante la maduración. Se identificó que FaβGal4 está implicado en la solubilización de pectinas a través de la liberación de galactosa, ... -
CRISPR/Cas9 editing of the polygalacturonase FaPG1 gene improves strawberry fruit firmness
López-Casado, Gloria; Sánchez-Raya, Cristina; Ric-Varas, Pablo; Paniagua Correas, Candelas; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Posé-Albacete, Sara; Matas, Antonio J.; Mercado-Carmona, José Ángel[et al.] (Oxford Academy, 2023-02-01)La firmeza es uno de los rasgos de calidad más importantes en las fresas. La vida útil postcosecha de esta fruta está altamente limitada por la pérdida de firmeza, donde la descomposición de la pared celular juega un papel ... -
Down-regulation of a pectin acetylesterase gene modifies strawberry fruit cell wall pectin stracture and increases fruit firmness
Matas-Arroyo, Antonio Javier; Rodríquez, Virginia; Sánchez, Luis; Paniagua Correas, Candelas; Pose-Albacete, Sara; García-Gago, Juan Antonio; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Mercado-Carmona, José Ángel[et al.] (2015-06-29)Antisense-mediated down-regulation of several fruit-specific genes has previously demonstrated how the cell wall disassembly in strawberry fruit is mediated by a series of enzymes that act sequentially (Posé et al. 2011). ... -
Efecto del silenciamiento de genes que codifican poligalacturonasas sobre el reblandecimiento del fruto de fresa asociado a la maduración
Paniagua Correas, Candelas; García-Gago, Juan Antonio; Matas-Arroyo, Antonio Javier; Barceló-Muñoz, Marta; Blanco-Portales, Rosario; Muñoz-Blanco, Juan; Pliego-Alfaro, Fernando; Quesada-Felice, Miguel Ángel; Mercado-Carmona, José Ángel[et al.] (2015-10-06)La degradación de las pectinas de la pared celular mediada por poligalacturonasas juega un papel clave en el reblandecimiento de la fresa. Así, el silenciamiento del gen FaPG1 incrementa la firmeza del fruto maduro y alarga ... -
Interactions between callose and cellulose revealed through the analysis of biopolymer mixtures
Abou-Saleh, Radwa H.; Hernandez-Gomez, Mercedes C.; Amsbury, Sam; Paniagua Correas, Candelas; Bourdon, Matthieu; Miyashima, Shunsuke; Helariutta, Ykä; Fuller, Martin; Budtova, Tatiana; Connell, Simon D.; Ries, Michael E.; Benitez-Alfonso, Yoselin; Paniagua Correas, Candelas[et al.] (Nature Research, 2018-10-31)Este estudio investigó las propiedades físico-mecánicas de los (1,3)-β-glucanos (calosa) mediante mezclas con celulosa en soluciones de líquidos iónicos e hidrogeles. Los resultados muestran que la adición de calosa reduce ... -
Nanostructural changes in cell wall pectins during strawberry fruit ripening assessed by atomic force microscopy
Paniagua Correas, Candelas; Kirby, Andrew R.; Gunning, A. Patrick; Morris, Victor J.; Matas-Arroyo, Antonio Javier; Quesada-Felice, Miguel Ángel; Mercado-Carmona, José Ángel[et al.] (2015-06-26)Rapid loss of firmness occurs during strawberry (Fragaria × ananassa Duch) ripening, resulting in a short shelf life and high economic losses. The disassembly of cell walls is considered the main responsible for fruit ... -
Nanostructural differences in pectic polymers isolated from strawberry fruits with low expression levels of pectate lyase or polygalacturonase genes
Pose-Albacete, Sara; Paniagua Correas, Candelas; Kirby, Andrew R.; Mercado-Carmona, José Ángel; Morris, Victor J.; Quesada-Felice, Miguel Ángel[et al.] (2013)Our research group has obtained transgenic strawberry plants expressing antisense sequences of either a pectate lyase (APEL lines) [1] or a polygalacturonase gene (APG lines) [2]. Both genes encode ripening-specific ... -
Polygalacturonase gene FaPG1 downregulation is related to increased strawberry fruit resistance to fungal decay
Paniagua Correas, Candelas; Sánchez-Raya, Cristina; Blanco-Portales, Rosario; Mercado-Carmona, José Ángel; Palomo-Ríos, Elena; Pose-Albacete, Sara[et al.] (2022-06-16)Plant health is a major target in breading programs because crops are under constant biotic stress, and climate change is exacerbating pests and disease negative impacts in agriculture. Obtaining crop varieties armed with ... -
Strawberry fruit softening and pectin disassembly: I. Nanostructural characterization of fruit pectins by atomic force microscopy II. Role of the β-Galactosidase gene FaβGal4
Paniagua Correas, Candelas (Universidad de Málaga, Servicio de Publicaciones y Divulgación Científica, 2014)La fresa se caracteriza por sufrir un rápido reblandecimiento durante el periodo de maduración, llegando a adquirir una textura semilíquida al final del desarrollo. Esta disminución de firmeza limita el periodo postcosecha ... -
Structural changes in cell wall pectins during strawberry fruit development
Santiago-Doménech, Nieves; Kirby, Andrew R.; Gunning, A. Patrick; Morris, Victor J.; Paniagua Correas, Candelas; Quesada-Felice, Miguel Ángel; Matas-Arroyo, Antonio Javier; Mercado-Carmona, José Ángel[et al.] (2017)Strawberry is one of the most important soft fruit. Rapid loss of firmness occurs during the ripening process, resulting in a short shelf life and high economic losses. To get insight into the role of pectin matrix in the ... -
Unravelling the nanostructure of strawberry fruit pectins by atomic force microscopy
Paniagua Correas, Candelas; Kirby, Andrew R.; Gunning, A. Patrick; Morris, Victor J.; Matas-Arroyo, Antonio Javier; Quesada-Felice, Miguel Ángel; Mercado-Carmona, José Ángel[et al.] (2016-07-01)Atomic force microscopy (AFM) allows the analysis of individual polymers at nanostructural level with a minimal sample preparation. This technique has been used to analyse the pectin disassembly process during the ripening ... -
Unravelling the nanostructure of strawberry fruit pectins by endo-polygalacturonase digestion and atomic force microscopy.
Paniagua Correas, Candelas; Kirby, Andrew R.; Gunning, A. Patrick; Morris, Victor J.; Matas-Arroyo, Antonio Javier; Quesada-Felice, Miguel Ángel; Mercado-Carmona, José Ángel[et al.] (Elsevier, 2016-12-19)Este estudio analizó las pectinas de las fresas utilizando microscopía de fuerza atómica (AFM) para visualizar sus cadenas y agregados. Las pectinas se trataron con una enzima específica, lo que resultó en una reducción ...