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Listar por autor "Alonso-Sanchez, Maria del Carmen"
Mostrando ítems 1-20 de 20
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Actividad antagonista de aislados del Virus de la Septicemia Hemorrágica Viral (VHSV) frente al sistema del interferón tipo I de lenguado senegalés
Gémez-Mata, Juan; Álvarez-Torres, Daniel; García-Rosado, Esther; Alonso-Sanchez, Maria del Carmen; Bejar-Alvarado, Julia (2017-10-11)Senegalese sole (Solea senegalensis) is susceptible to marine Viral Haemorrhagic Septicaemia Virus (VHSV) isolates, but it is not affected by freshwater VHSV isolates. In addition, the sole type I interferon (IFN I) response ... -
Antiviral activity of European sea bass ISG15 against betanodavirus infections
Moreno, Patricia; García-Rosado, Esther; Borrego-Garcia, Juan Jose; Alonso-Sanchez, Maria del Carmen (2017)To study the role of the sea bass ISG15 protein against betanodavirus infections, an in vivo transcription analysis has been performed after RGNNV infection and poly I:C inoculation, and the sea bass ISG15 activity has ... -
ANTIVIRAL ACTIVITY OF MX PROTEINS FROM GILTHEAD SEABREAM (SPARUS AURATA) AGAINST LYMPHOCYSTIS DISEASE VIRUS (LCDV)
Fernández-Trujillo, Alejandra; García-Rosado, Esther; Alonso-Sanchez, Maria del Carmen; Borrego-Garcia, Juan Jose; Alvarez-Herrero, Maria del Carmen; [et al.] (ELSEVIER, 2013)Gilthead seabream (Sparus aurata), one of the most important fish species in the Mediterranean Aquaculture displays a high natural resistance to viral diseases. Until now, the main viral disease affecting gilthead seabream ... -
Betanodavirus virulence to European sea bass (Dicentrarchus labrax): a new insight into virus-host interaction
Moreno Garcia, Patricia (UMA Editorial, 2018-12-21)Viral diseases are the result of the pathogen virulence, the host immunity mechanisms, and the interaction between those components, which constitutes the so called host-pathogen interaction. This PhD Thesis is a comprehensive ... -
Characterization of Senegalese sole (Solea senegalensis) Mx promoter
Carballo, Carlos; Alonso-Sanchez, Maria del Carmen; Collet, Bertrand; Bejar-Alvarado, Julia; Álvarez-Torres, Daniel; [et al.] (Elsevier, 2013)Type I interferon (IFN) represents a first component of the innate immune response against viral infections, promoting an antiviral state in cells. Mx proteins are the best studied IFN-inducible genes (ISGs) in fish. The ... -
Development of in vitro systems to study IFN signalling in gilthead seabream (Sparus aurata)
González-Mariscal, J.A.; Fernández-Trujillo, M.A.; Méndez, T.; Alonso-Sanchez, Maria del Carmen; García-Rosado, Esther; [et al.] (2014-12-11)Type I interferon (IFN I) triggers specific signalling pathways leading to the activation of the innate immune defence of vertebrates against viral infections. In contrats, type II IFN (IFN II) is generally accepted to be ... -
Differences in the viral induction of the senegalese sole Mx protein in RTG-2 and CHSE-214 cells
Álvarez-Torres, Daniel; Podadera, Ana Maria; García-Rosado, Esther; Collet, Bertran; Bejar-Alvarado, Julia; [et al.] (EAFP, 2013)Transfected RTG-2 cells infected with VHSV showed significant induction of the luciferase reporter gene, compared to the control non-infected cells, at 24, 48 and 72 h post infection (p.i.). The maximum expression was ... -
Differential induction of the gilthead seabream Mx1, Mx2 and Mx3 promoters by IPNV and VHSV
González-Mariscal, J.A.; Fernández-Trujillo, M.A.; Méndez, T.; Alonso-Sanchez, Maria del Carmen; García-Rosado, Esther; [et al.] (2014-10-07)Type I interferon (IFN I) system triggers specific signalling pathways leading to the activation of the innate immune defence of vertebrates against viral infections. The complex expression regulation of Interferon Stimulated ... -
Estimulación del sistema mediado por interferón tipo I de lenguado senegalés (Solea senegalensis) en respuesta a infecciones por nodavirus
Podadera, AM; Álvarez-Torres, Daniel; Souto, S; Bandin, I; Alonso-Sanchez, Maria del Carmen (2014-09-25)El lenguado senegalés es susceptible a la infección por el Virus de la Necrosis Nerviosa Viral (VNNV). Los betanodavirus se clasifican en cuatro genotipos, siendo los genotiposSJNNV, RGNNV y virus recombinantes RGNNV-SJNNV ... -
Gilthead seabream (Sparus aurata) Mx proteins show positive and negative synergy in their antiviral activity
Fernández-Trujillo, M.A.; García-Rosado, Esther; Alonso-Sanchez, Maria del Carmen; Borrego-Garcia, Juan Jose; Alvarez-Herrero, Maria del Carmen; [et al.] (2014-10-07)Due to their direct antiviral activity, Mx proteins play a main role in the response mediated by the type I interferon against viral infections. The study of the farmed fish gilthead seabream Mx genes is specially interesting, ... -
In vitro viral coexistence decreases Striped Jack Nervous Necrosis Virus (SJNNV) but favours Red-spotted Grouper Nervous Necrosis Virus (RGNNV) replication
López Jimena, Benjamín; Alonso-Sanchez, Maria del Carmen; Infante, Carlos; Castro, Dolores; Borrego-Garcia, Juan Jose; [et al.] (European Association of Fish Pathologists, 2013)In this study, several assays have been performed to characterise the effect of the RGNNV-SJNNV coexistence (coinfection and superinfection) on the replication of each genotype on E-11 cells.The results obtained showed the ... -
Infection of Artemia sp. by Lymphocystis disease virus (LCDV)
Valverde Jiménez, Estefanía; Cano, Irene; García-Rosado, Esther; Alonso-Sanchez, Maria del Carmen; Borrego-Garcia, Juan Jose; [et al.] (2013-12-10)In the present work, different developmental stages of Artemia sp. (metanauplius, juvenile and adult) were experimentally infected with LCDV by immersion. Results of viral quantification (both by qPCR and cell-culture viral ... -
Papel del inmunogen isg15 de lubina (Dicentrarchus labrax) en las infecciones por betanodavirus
Moreno, Patricia; García-Rosado, Esther; Borrego-Garcia, Juan Jose; Alonso-Sanchez, Maria del Carmen (Universidad de Oviedo, 2016)En peces, al igual que en vertebrados superiores, la respuesta antiviral innata está mediada por el sistema interferón tipo I (IFN I), que actúa mediante la activación de genes denominados isg (interferon-stimulated genes), ... -
RRGNNV capsid protein amino acids 247 and 270 are involved in betanodavirus virulence to European sea bass (Dicentrarchus labrax)
Moreno, Patricia; Souto, Sandra; Leiva-Revollo, Rocio; García-Rosado, Esther; Borrego-Garcia, Juan Jose; [et al.] (2019-09-18)Recombinant RGNNV viruses harbouring SJNNV-type amino acids at positions 247 and/or 270 (Mut247Dl965, Mut270Dl965, Mut247+270Dl965) were generated by reverse genetics. The effect of these modifications on viral replication ... -
Senegalese sole (Solea senegalensis) ISG15: molecular characterization and in vivo interplay with viral infections
Álvarez-Torres, Daniel; Podadera, Ana María; Bandin, Isabel; Bejar-Alvarado, Julia; García-Rosado, Esther; [et al.] (2015-09-17)The interferon-stimulated gene 15 (Isg15) is strongly induced by type I interferon (IFN I), viral infection, and double-stranded RNA (poly I:C) in several fish species, suggesting that Isg15 protein could play a key role ... -
Senegalese sole transcriptomic profiles in response to different betanodavirus RGNNV/SJNNV reassortant strains
Labella, Alejandro; Borrego-Garcia, Juan Jose; Manchado, Manuel; Bandín, Isabel; Castro, Dolores; [et al.] (2017)Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a disease that has been reported in more than 40 marine and freshwater fish species worldwide, including Senegalese sole (Solea senegalensis). Based ... -
El sistema del interferón tipo I protege a juveniles de lenguado senegalés (Solea senegalensis) frente a la infección por VHSV
Álvarez-Torres, Daniel; Podadera, AM; Vieytes, LP; Souto, S; Bandin, S; [et al.] (2014-09-25)El lenguado senegalés (Solea senegalensis) es susceptible a la infección por el Virus de la Septicemia Hemorrágica Viral (VHSV) bajo condiciones experimentales. El objetivo de este trabajo es determinar el papel del sistema ... -
Susceptibility of juvenile european sea bass (dicentrarchus labrax) to different viral nervousnecrosis virus (VNNV) isolates
Lopez-Jimena, Benjamin; García-Rosado, Esther; Souto-Pereira, Sandra; Infante, Carlos; Borrego-Garcia, Juan Jose; [et al.] (EAFP, 2013-09-02)In this study, the susceptibility of 5-g juvenile European sea bass was evaluated by intramuscular injection (105 TCID50/g) using isolates belonging to the RGNNV and SJNNV genotypes as well as a reassortant isolate ... -
The Senegalese Sole Mx gene promoter contains a variable microsatellite region involved in the transcriptional
Álvarez-Torres, Daniel; Vieytes, Lisandro P; García-Rosado, Esther; Alonso-Sanchez, Maria del Carmen; Bejar-Alvarado, Julia (2015-09-14)Interferons (IFNs) play a key role against viral infections by stimulating the expression of IFN stimulated genes, such as Mx. The transcriptional regulation of these genes in fish are poorly understood. The sequencing ... -
The stimulation of the innate immune system by SJNNV protects juvenile european sea bass (Dicentrarchus labrax) against superinfection with RGNNV
Carballo, Carlos; López-Jimena, Benjamin; Álvarez-Torres, Daniel; García-Rosado, Esther; Borrego-Garcia, Juan Jose; [et al.] (2013-12-04)In this study, viral replication and transcription of innate immunogenes have been determined by RT-real time-PCR (RT-qPCR) in the course of an experimental infection. Three different experimental conditions were considered: ...