Listar por autor "Amorim-Silva, Vitor"
Mostrando ítems 1-20 de 31
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Adaptative mechanisms of cellulose synthesis under stress conditions
Pagano Márquez, Raquel; Macho, Alberto P.; Castillo-Garriga, Araceli
; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel (2023-07-09)
Cellulose is the main structural compound of the plant cell wall and the most abundant biopolymer on Earth (Bar‐On et al 2018). The essential role of cellulose in plant development and defence highlights the importance ... -
Arabidopsis NahG Plants as a Suitable and Efficient System for Transient Expression using Agrobacterium tumefaciens
Rosas-Díaz, Tábata Victoria; Cana Quijada, José Francisco; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel; Lozano Durán, Rosa; Bejarano, Eduardo R.[et al.] (Science direct, 2017-02)
aken together, our results show that expression of the NahG transgene in Arabidopsis dramatically enhances the efficiency of Agrobacterium-mediated transformation in rosette leaves, enabling the routine use of this technique ... -
Arabidopsis Squalene Epoxidase 3 (SQE3) Complements SQE1 and Is Important for Embryo Development and Bulk Squalene Epoxidase Activity
Laranjeira, Sara; Amorim-Silva, Vitor; Esteban del Valle, Alicia; Arró, Montserrat; Ferrer, Albert; Tavares, Rui M.; Botella-Mesa, Miguel Ángel; Rosado, Abel; Azevedo, Herlânder[et al.] (ScienceDirect, 2015-07-06)
The existence of multigenic families in the mevalonate pathway suggests divergent functional roles for pathway components involved in the biosynthesis of plant sterols. Squalene epoxidases (SQEs) are key components of this ... -
Arabidopsis Synaptotagmins 1 and 3 are involved in lipid homeostasis at ER-PM contact sites under cold stress
Amorim-Silva, Vitor; Esteban del Valle, Alicia; Castillo-Garriga, Araceli; Rosado Rey, Abel; Vanneste, Steffen; Perea, Carlos; Salinas, Julio; Botella-Mesa, Miguel Ángel; Ruiz-López, Noemí; Pérez-Sancho, Jessica; Schapire, Arnaldo; Osorio-Algar, Sonia; Willmitzer, Lothar[et al.] (2018-07-12)
As sessile organisms, plants must cope with abiotic stress such as soil salinity, drought, and extreme temperatures. This stress signal can activate a phospholipase C (PLC), which hydrolyses PIP2 to generate IP3 and ... -
Calling for reinforcements: the role of TTL proteins in the regulación of cell wall biosynthesis
Percio-Vargas, Francisco; Kesten, Christopher; García-Moreno, Álvaro; Amorim-Silva, Vitor; Castillo-Garriga, Araceli; Ruiz-López, Noemí; Sánchez-Rodríguez, Clara; Botella-Mesa, Miguel Ángel[et al.] (2022-09-14)
Cellulose is the most abundant organic compound of all biomass on Earth1, with highly relevant roles in plant development and defence. Hence, it is essential to understand the regulation of its biosynthesis to improve the ... -
Crucial Roles of Brassinosteroids in Cell Wall Composition and Structure Across Species: New Insights and Biotechnological Applications
Percio-Vargas, Francisco; Rubio-Valverde, Lourdes; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel (Wiley, 2024)
Brassinosteroids (BR) are steroidal phytohormones essential for plant growth, development, and stress resistance. They fulfil this role partially by modulating cell wall structure and composition through the control of ... -
Diacylglycerol transport by Arabidopsis Synaptotagmin 1 at ERplasma membrane contact sites under abiotic stress.
Ruiz-López, Noemí; Pérez-Sancho, Jessica; Esteban del Valle, Alicia; García-Hernández, Selene; Huércano Rubens, Carolina; Percio-Vargas, Francisco; Benítez de la Fuente, Francisco; Osorio-Algar, Sonia; Steffen, Vanneste; Lothar, Willmitzer; Napier, Johnathan A.; Perea, Carlos; Salinas, Julio; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel[et al.] (2019-11-13)
Bulk lipid transport between membranes within cells involves vesicles, however membrane contact sites have recently been discovered as mediators of non-vesicular lipid transfer. ER-PM contact sites are conserved structures ... -
Effect of salt on ROS homeostasis, lipid peroxidation and antioxidant mechanisms in Pinus pinaster suspension cells.
(INRA EDP Sciences, 2009-01)In the Pinus genus, information on the effectiveness of oxidative defence mechanisms during exposure to salt is lacking. The effect of salt stress imposition on ROS homeostasis was investigated using maritime pine (Pinus ... -
Functional characterization of Arabidopsis thaliana Synaptotagmin1 domains using Tricalbin3 chimeras in Saccharomyces cerevisiae
Benítez-Fuente, Francisco; Amorim-Silva, Vitor; Collado, Javier; Fernández-Busnadiego, Rubén; Botella-Mesa, Miguel Ángel (2023-06)
Synaptotagmin1 (SYT1) is an Arabidopsis thaliana endoplasmic reticulum (ER)-plasma membrane contact site tether involved in biotic and abiotic stress resistance. These resistance roles have been related to SYT1 tethering ... -
Genetic and genome-wide transcriptomic analyses identify co- regulation of oxidative response and hormone transcript abundance with vitamin C content in tomato fruit.
Lima-Silva, Viviana; Rosado, Abel; Amorim-Silva, Vitor; Muñoz-Mérida, Antonio; Pons, Clara; Bombarely, Aureliano; Trelles, Oswaldo; Fernández Muñoz, Rafael; Granell, Antonio; Valpuesta-Fernández, Victoriano; Botella-Mesa, Miguel Ángel[et al.] (BioMed Central, 2012-05-14)
L-ascorbic acid (AsA; vitamin C) is essential for all living plants where it functions as the main hydrosoluble antioxidant. It has diverse roles in the regulation of plant cell growth and expansion, photosynthesis, and ... -
Genetic determinants of Vitamin C content in higher plants
Fenech-Torres, Mario; Amorim-Silva, Vitor; Smirnoff, Nicholas; Valpuesta-Fernández, Victoriano; Botella-Mesa, Miguel Ángel (2018-07-18)
Ascorbic acid (AsA, VitC) is the most abundant water-soluble antioxidant in plants and it plays a plethora of biological roles including resistance to abiotic stress. Hence, including VitC as a trait to improve in breeding ... -
Identification of SYT1 interactors connects calcium signaling, endoplasmic reticulum bending, plasmodesmata and membrane contact sites.
Benítez de la Fuente, Francisco; Pérez-Sancho, Jessica; Huércano Rubens, Carolina; Amorim-Silva, Vitor; Macho, Alberto P.; Bayer, Emmanuelle M.; De Jaeger, Geert; Ruiz-López, Noemí; Botella-Mesa, Miguel Ángel[et al.] (2021)
Synaptotagmin1 (SYT1) is an Arabidopsis thaliana protein essential for tolerance to several abiotic stresses (Schapire et al., 2008; Pérez-Sancho et al., 2015; Ruiz-Lopez et al., 2020). SYT1 forms endoplasmic reticulum-plasma ... -
Molecular Characterization of ZosmaNRT2, the Putative Sodium Dependent High-Affinity Nitrate Transporter of Zostera marina L.
Rubio-Valverde, Lourdes; Días-García, Jordi; Amorim-Silva, Vitor; Macho, Alberto P.; Botella-Mesa, Miguel Ángel; Fernández-García, José Antonio[et al.] (MDPI, 2019-07-26)
One of the most important adaptations of seagrasses during sea colonization was the capacity to grow at the low micromolar nitrate concentrations present in the sea. In contrast to terrestrial plants that use H+ symporters ... -
NAHG increases transient transformation of arabidopsis leaves by agrobacterium
Cana Quijada, José Francisco; Rosas-Díaz, Tábata Victoria; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel; Lozano-Durán, Rosa; Rodríguez-Bejarano, Eduardo[et al.] (2018-07-11)
Agrobacterium tumefaciens-mediated transient transformation has demonstrated to be an invaluable tool in plant molecular biology studies. However, low efficiency and inconsistency of this method in Arabidopsis has forced ... -
Overcoming stress: new insights in the regulation of cell wall biosynthesis
Sánchez-Rodríguez, Clara; Percio-Vargas, Francisco; Kesten, Christopher; García-Moreno, Álvaro; Amorim-Silva, Vitor; Castillo-Garriga, Araceli; Ruiz-López, Noemí; Botella-Mesa, Miguel Ángel[et al.] (2022)
In addition to being crucial in plant development and defence, cellulose is the most abundant organic compound of all biomass on Earth1. Therefore, it is essential to elucidate the regulation of its biosynthesis to improve ... -
Peripheral membrane proteins modulate stress tolerance by safeguarding cellulose synthases
Kesten, Christopher; García-Moreno, Álvaro; Amorim-Silva, Vitor; Menna, Alexandra; Castillo-Garriga, Araceli; Percio, Francisco; Armengot, Laia; Ruiz-López, Noemí; Jaillais, Yvon; Sánchez-Rodríguez, Clara; Botella-Mesa, Miguel Ángel[et al.] (American Association for the Advancement of Science, 2022-11-16)
Controlled primary cell wall remodeling allows plant growth under stressful conditions, but how these changes are conveyed to adjust cellulose synthesis is not understood. Here, we identify the TETRATRICOPEPTIDE THIOREDOXIN-LIKE ... -
Peripheral membrane TTL proteins safeguard cellulose synthesis under stress.
Amorim-Silva, Vitor; García-Moreno, Álvaro; Kesten, Christopher; Menna, Alexandra; Castillo-Garriga, Araceli; Percio-Vargas, Francisco; Pagano Márquez, Raquel; Moya-Cuevas, José; Armengot, Laia; Ruiz-López, Noemí; Jallais, Yvon; Sánchez-Rodríguez, Clara; Botella-Mesa, Miguel Ángel[et al.] (2023)
Land plants provide around eighty percent of biomass on Earth and roughly one-third corresponds to cellulose (Bar-On et al 2018). Despite its biological and societal importance, many aspects of cellulose biosynthesis and ... -
Phosphorylation regulates cellulose biosynthesis regulation.
Percio-Vargas, Francisco; Toth, Ryan; DeFalco, Thomas A.; Persson, Staffan; Zipfel, Cyril; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel[et al.] (2023)
Plants comprise over 82% of all biomass on Earth, a third of which is cellulose, making it the most abundant organic compound¹. Cellulose is also essential for plant development and defense against multiple stresses. ... -
REGULATION OF VITAMIN C BIOSYNTHESIS IN HIGHER PLANTS
Fenech-Torres, Mario; Amorim-Silva, Vitor; Castillo-Garriga, Araceli; Esteban del Valle, Alicia; Valpuesta-Fernández, Victoriano; Smirnoff, Nicholas; Botella-Mesa, Miguel Ángel[et al.] (2019-05-15)
Ascorbate, also known as vitamin C, plays fundamental roles in biotic and abiotic stress resistance in plants. In green tissues, ascorbate is mainly synthesized through the Smirnoff-Wheeler pathway, whose regulation is not ... -
Regulation of Vitamin C content in higher plants
Fenech-Torres, Mario; Amorim-Silva, Vitor; Smirnoff, Nicholas; Botella-Mesa, Miguel Ángel (2017-06-23)
Ascorbic Acid (AsA, vitamin C) has multiple biological roles in plants. Although several pathways for the novo synthesis of AsA have been reported, it is the L-Gal, also known as the Smirnoff-Wheeler (SW), the main pathway ...