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Listar por autor "García-Rosado, Esther"
Mostrando ítems 21-39 de 39
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Immunogene expression analysis in betanodavirus infected-Senegalese sole using an OpenArray® platform.
Gémez-Mata, Juan; Labella Vera, Alejandro Manuel; Bandín Matos, Isabel; Borrego-García, Juan José; García-Rosado, Esther (ELSEVIER, 2021-03-30)The transcriptomic response of Senegalese sole (Solea senegalensis) triggered by two betanodaviruses with different virulence to that fish species has been assessed using an OpenArray® platform based on TaqMan™ quantitative ... -
In vitro viral coexistence decreases Striped Jack Nervous Necrosis Virus (SJNNV) but favours Red-spotted Grouper Nervous Necrosis Virus (RGNNV) replication
López-Jimena, Benjamín; Alonso-Sánchez, María del Carmen; Infante, Carlos; Castro-López, María Dolores; Borrego-García, Juan José; García-Rosado, Esther[et al.] (European Association of Fish Pathologists, 2013)In this study, several assays have been performed to characterise the effect of the RGNNV-SJNNV coexistence (coinfection and superinfection) on the replication of each genotype on E-11 cells.The results obtained showed the ... -
Infection of Artemia sp. by Lymphocystis disease virus (LCDV)
Jiménez Valverde, Estefanía; Cano, Irene; García-Rosado, Esther; Alonso-Sánchez, María del Carmen; Borrego-García, Juan José; Castro-López, María Dolores[et al.] (2013-12-10)In the present work, different developmental stages of Artemia sp. (metanauplius, juvenile and adult) were experimentally infected with LCDV by immersion. Results of viral quantification (both by qPCR and cell-culture viral ... -
Inhibition of nervous necrosis virus replication by Shewanella putrefaciens Pdp11 extract
Moreno, Patricia; Álvarez-Torres, Daniel; Balebona-Accino, María del Carmen; Domínguez-Maqueda, Marta; Moriñigo-Gutiérrez, Miguel Ángel; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen; García-Rosado, Esther[et al.] (Elsevier, 2023)One of the most relevant infectious diseases affecting farmed fish is the viral encephalopathy and retinopathy, caused by the nervous necrosis virus (NNV). The two commercial vaccines available against this virus have been ... -
Mx Protein involvement in the innate immune system against viral infections in Senegalese Sole (Solea senegalensis)
Álvarez-Torres, Daniel (Universidad de Málaga, Servicio de Publicaciones y Divulgación Científica, 2014)La acuicultura es una actividad de gran interés económico y ecológico, que presenta un alto potencial de crecimiento, y cuyo desarrollo sostenible constituye la vía óptima de aprovechamiento de los recursos pesqueros sin ... -
Papel del inmunogen isg15 de lubina (Dicentrarchus labrax) en las infecciones por betanodavirus
Moreno, Patricia; García-Rosado, Esther; Borrego-García, Juan José; Alonso-Sánchez, María del Carmen (Universidad de Oviedo, 2016)En peces, al igual que en vertebrados superiores, la respuesta antiviral innata está mediada por el sistema interferón tipo I (IFN I), que actúa mediante la activación de genes denominados isg (interferon-stimulated genes), ... -
Polisacáridos de Porphyridium cruentum presentan actividad antiviral diferencial frente a infecciones causadas de VHSV y VNN
Moreno Garcia, Patricia; Parra-Riofrio, Geovanna; Abdala-Díaz, Roberto Teófilo; García-Rosado, Esther; Uribe-Tapia, Eduardo; Alonso-Sánchez, María del Carmen; Béjar-Alvarado, Julia[et al.] (Sociedad Española de Microbiología, 2021)Los microorganismos del medio marino constituyen una importante fuente de compuestos cuya utilización en la prevención y/o tratamiento de enfermedades de etiología viral está siendo sugerida en los últimos años. En concreto, ... -
RRGNNV capsid protein amino acids 247 and 270 are involved in betanodavirus virulence to European sea bass (Dicentrarchus labrax)
Moreno, Patricia; Souto, Sandra; Leiva-Rebollo, Rocío; García-Rosado, Esther; Borrego-García, Juan José; Bandín Matos, Isabel; Alonso-Sánchez, María del Carmen[et al.] (2019-09-18)Recombinant RGNNV viruses harbouring SJNNV-type amino acids at positions 247 and/or 270 (Mut247Dl965, Mut270Dl965, Mut247+270Dl965) were generated by reverse genetics. The effect of these modifications on viral replication ... -
Senegalese sole (Solea senegalensis) ISG15: molecular characterization and in vivo interplay with viral infections
Álvarez-Torres, Daniel; Podadera, Ana María; Bandín Matos, Isabel; Béjar-Alvarado, Julia; García-Rosado, Esther; Alonso-Sánchez, María del Carmen[et al.] (2015-09-17)The interferon-stimulated gene 15 (Isg15) is strongly induced by type I interferon (IFN I), viral infection, and double-stranded RNA (poly I:C) in several fish species, suggesting that Isg15 protein could play a key role ... -
Senegalese sole immune response against betanodavirus recombinants harboring modifications in the 3' terminal region of the RNA1
Gémez-Mata, Juan; Labella Vera, Alejandro Manuel; Souto, Sandra; Bandín Matos, Isabel; Borrego-García, Juan José; García-Rosado, Esther[et al.] (2021-09)The nervous necrosis virus (NNV) is the etiological agent of the viral nervous necrosis (VNN), a disease affecting a high number of fish species worldwide. NNV genome is composed of two segments RNA1 and RNA2, encoding the ... -
Senegalese sole transcriptomic profiles in response to different betanodavirus RGNNV/SJNNV reassortant strains
Labella Vera, Alejandro Manuel; Borrego-García, Juan José; Manchado, Manuel; Bandín Matos, Isabel; Castro-López, María Dolores; Alonso-Sánchez, María del Carmen; García-Rosado, Esther[et al.] (2017)Betanodaviruses are the causative agents of viral nervous necrosis (VNN), a disease that has been reported in more than 40 marine and freshwater fish species worldwide, including Senegalese sole (Solea senegalensis). Based ... -
Shewanella putrefaciens Pdp11 extracts protect against betanodavirus infection.
Moreno-García, Patricia; Álvarez-Torres, Daniel; Balebona-Accino, María del Carmen; Moriñigo-Gutiérrez, Miguel Ángel; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen; García-Rosado, Esther[et al.] (2023)Viral nervous necrosis is caused by the nervous necrosis virus (Betanodavirus genus), a naked virus with two positive-sense RNA segments. Betanodaviruses are classified into four species, being RGNNV predominant in the ... -
El sistema del interferón tipo I protege a juveniles de lenguado senegalés (Solea senegalensis) frente a la infección por VHSV
Álvarez-Torres, Daniel; Podadera, AM; Vieytes, Lisandro P; Souto, S; Bandin, S; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen; García-Rosado, Esther[et al.] (2014-09-25)El lenguado senegalés (Solea senegalensis) es susceptible a la infección por el Virus de la Septicemia Hemorrágica Viral (VHSV) bajo condiciones experimentales. El objetivo de este trabajo es determinar el papel del sistema ... -
Susceptibility of juvenile european sea bass (dicentrarchus labrax) to different viral nervousnecrosis virus (VNNV) isolates
López-Jimena, Benjamín; García-Rosado, Esther; Souto-Pereira, Sandra; Infante, Carlos; Borrego-García, Juan José; Alonso-Sánchez, María del Carmen[et al.] (EAFP, 2013-09-02)In this study, the susceptibility of 5-g juvenile European sea bass was evaluated by intramuscular injection (105 TCID50/g) using isolates belonging to the RGNNV and SJNNV genotypes as well as a reassortant isolate ... -
Tetraselmis suecica and Porphyridium cruentum exopolysaccharides show anti-VHSV activity on RTG-2 cells
Parra-Riofrio, Geovanna; Moreno, Patricia; García-Rosado, Esther; Alonso-Sánchez, María del Carmen; Uribe-Tapia, Eduardo; Abdala-Díaz, Roberto Teófilo; Béjar-Alvarado, Julia[et al.] (Springer, 2023)The use of functional feed additives is an important approach to both, prevent and fight, viral diseases in aquaculture. In this regard, microalgae-derived products, and, more specifically, microalgal exopolysaccharides ... -
The Senegalese Sole Mx gene promoter contains a variable microsatellite region involved in the transcriptional
Álvarez-Torres, Daniel; Vieytes, Lisandro P; García-Rosado, Esther; Alonso-Sánchez, María del Carmen; Béjar-Alvarado, Julia (2015-09-14)Interferons (IFNs) play a key role against viral infections by stimulating the expression of IFN stimulated genes, such as Mx. The transcriptional regulation of these genes in fish are poorly understood. The sequencing ... -
The stimulation of the innate immune system by SJNNV protects juvenile european sea bass (Dicentrarchus labrax) against superinfection with RGNNV
Carballo Perez, Carlos; López-Jimena, Benjamín; Álvarez-Torres, Daniel; García-Rosado, Esther; Borrego-García, Juan José; Alonso-Sánchez, María del Carmen[et al.] (2013-12-04)In this study, viral replication and transcription of innate immunogenes have been determined by RT-real time-PCR (RT-qPCR) in the course of an experimental infection. Three different experimental conditions were considered: ... -
Transcriptional profile and serological studies of the European sea bass immune response against betanodavirus infections
Moreno, Patricia; López-Jimena, Benjamín; Scapigliati, Giuseppe; Buonocuore, Francesco; García-Rosado, Esther; Borrego-García, Juan José; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen[et al.] (2019-07-19)European sea bass is highly affected by outbreaks of viral nervous necrosis disease, provoked by the nervous necrosis virus (NNV). This virus displays a positive-sense RNA genome composed of two segments, RNA1, encoding ... -
Validation of two pmx-luciferase reporter systems to study virus - host interaction.
Béjar-Alvarado, Julia; Álvarez-Torres, Daniel; Alonso-Sánchez, María del Carmen; García-Rosado, Esther (2023)Viral diseases represent an important threat in aquaculture. Therefore, the development of strategies and tools to understand fish susceptibility, virus-host interaction, or to identify viral virulence markers is a priority. ...