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dc.contributor.authorGarcía-Bonilla, María
dc.contributor.authorCifuentes-Rueda, Manuel 
dc.contributor.authorHo-Plágaro, Ailec
dc.contributor.authorShumilov, Kirill
dc.contributor.authorSantos-Ruiz, Leonor 
dc.contributor.authorPérez-Fígares, José Manuel
dc.contributor.authorJiménez-Lara, Antonio Jesús 
dc.date.accessioned2015-07-08T12:01:09Z
dc.date.available2015-07-08T12:01:09Z
dc.date.created2015
dc.date.issued2015-07-08
dc.identifier.urihttp://hdl.handle.net/10630/10059
dc.description.abstractBone marrow-derived mesenchymal stem cells (BM-MSC) are considered as a potential therapeutic tool in neurodegenerative diseases, due to their ability to migrate to degenerated tissues and the production of growth factors. Congenital hydrocephalus is a disorder characterized by a degeneration of the periventricular cerebral parenchyma and the white matter. In the present study, using an animal model of congenital hydrocephalus, the hyh mouse, it has been studied the capacity of the BM-MSC to reach the degenerated regions exhibiting glial reactions and their probable neuroprotector effects. The BM-MSC were isolated from two sources: a) transgenic mice expressing the monomeric red fluorescent protein (mRFP1); b) wild type mice. In the second case, the BM-MSC were labelled in vitro using bromodeoxyuridine, a fluorescent cell tracker and the lipophilic DiR. Before application, the cells were analysed using flow cytometry and immunofluorescence. The BM-MSC were injected into the retro-orbital sinus or into the lateral ventricle of hyh mice. After 24/96 hours of administration, they were detected under light, confocal and electron microscopes. The injected BM-MSC reached the degenerated periventricular regions and the disrupted neurogenic niches. They were detected in the periventricular parenchyma, around periventricular blood vessels and in the ventral meninges. Most of the applied BM-MSC expressed the glial cell-derived neurotrophic factor (GDNF), in the same way as the periventricular reactive astrocytes, suggesting a possible neuroprotector effect.es_ES
dc.description.sponsorshipUniversidad de Málaga, Campus de Excelencia Internacional Andalucía Tech. Instituto de Salud Carlos III, PI12/0631 con cofinanciación FEDER.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectHidrocefaliaes_ES
dc.subject.otherMesenchymal stem cellses_ES
dc.subject.otherHydrocephaluses_ES
dc.titleCharacterization and administration of bone marrow-derived mesenchymal stem cells in an animal model of congenital hydrocephaluses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXVI Congreso Sociedad Española de Biología Celulares_ES
dc.relation.eventplaceSevillaes_ES
dc.relation.eventdate29/06/2015es_ES
dc.rights.ccby-nc-nd


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