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dc.contributor.authorRodríguez-Negrete, Edgar
dc.contributor.authorPiedra-Aguilera, Álvaro
dc.contributor.authorLozano-Durán, Rosa
dc.contributor.authorCruzado, Lucia
dc.contributor.authorRodríguez-Bejarano, Eduardo 
dc.contributor.authorCastillo-Garriga, Araceli 
dc.date.accessioned2015-10-20T07:14:16Z
dc.date.available2015-10-20T07:14:16Z
dc.date.created2015
dc.date.issued2015-10-20
dc.identifier.urihttp://hdl.handle.net/10630/10543
dc.description.abstractViruses are masters at circumventing host defenses and manipulating the cellular environment for their own benefit. The replication of the largest known family of single-stranded DNA viruses, Geminiviridae, is impaired by DNA methylation but the fact that plants might use methylation as a defense against geminiviruses and the impact that viral genome methylation may have during the infection, remain controversial. We have found that geminiviruses reduce the expression of the plant maintenance DNA methyltransferases, MET1 and CMT3, in both, locally and systemically infected tissues. Furthermore, we demonstrated that the virus-mediated repression of these two maintenance DNA methyltransferases is widely spread among different geminivirus species and we have identified Rep as the geminiviral protein responsible for the repression of MET1 and CMT3. The presence of Rep, suppresses transcriptional gene silencing (TGS) of an Arabidopsis transgene and of host loci whose expression is strongly controlled by MET1. Bisulfite sequencing analyses showed that the expression of Rep caused a substantial reduction in the levels of DNA methylation at certain loci at CG sites. The biological relevance of these findings and the role of Rep as a TGS suppressor will be discussed.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectInactivación génicaes_ES
dc.subject.otherDNA methylationes_ES
dc.subject.otherRepes_ES
dc.subject.otherGeminiviruses_ES
dc.subject.otherEpigeneticses_ES
dc.subject.otherGene silencinges_ES
dc.titleGeminivirus Rep Protein Interferes with the Plant DNA Methylation Machinery and Suppresses Transcriptional Gene Silencinges_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXL Congreso de la Sociedad Española de Genéticaes_ES
dc.relation.eventplaceCórdobaes_ES
dc.relation.eventdate16-9-2015es_ES
dc.cclicenseby-nc-ndes_ES


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