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dc.contributor.authorCana Quijada, José Francisco
dc.contributor.authorRosas-Díaz, Tábata Victoria
dc.contributor.authorLozano Durán, Rosa
dc.contributor.authorRodríguez-Bejarano, Eduardo 
dc.date.accessioned2015-10-21T11:08:53Z
dc.date.available2015-10-21T11:08:53Z
dc.date.created2015
dc.date.issued2015-10-21
dc.identifier.urihttp://hdl.handle.net/10630/10556
dc.description.abstractTomato yellow leaf curl Sardinian virus (TYLCSV) is one of the causal agent of the tomato yel-low leaf curl disease, one of the most important threats to tomato crops worldwide. TYLCSV is a monopartite member of the genus Begomovirus from the family Geminiviridae. To carry out a full infection, geminiviruses need to move inside the infected cell and from one cell to an-other for which they depend on diverse cellular factors. While cell-to-cell movement has been described to occur through plasmodesmata, the way in which geminiviruses move inside the host cells is yet unknown. The identification of the host proteins involved in viral infection will be an important step to-wards the understanding of the mechanisms underlying this process. In our laboratory, trans-genic Nicotianabenthamiana plants containing a green fluorescent protein (GFP) expression cassette flanked by two direct repeats of the intergenic region of TYLCSV have been construct-ed (2IR plants). When these plants are infected with TYLCSV, an overexpression of the reporter gene is observed in those cells where the virus replicates. These plants have been used to-gether with virus induced gene silencing (VIGS) in an effort to identify host genes involved in the infection process using a reverse genetics approach. Using this combined technique our group has identified two genes δ-COP and ARF 1, involved in retrograde vesicle trafficking, which are essential for the infectious process. We are current-ly assaying genes codifying proteins involved in different pathways of the vesicle trafficking system: Sar1b, γ subunit of AP1, Sec24, SYT1 and two that encode the heavy chain of triskelion proteins. Their effect over virus infection will be presented and discussed.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectVirus fitopatógenoses_ES
dc.subject.otherVIGSes_ES
dc.subject.otherTYLCSVes_ES
dc.subject.otherCOPes_ES
dc.subject.otherVesicle traffickinges_ES
dc.subject.otherGeminiviruses_ES
dc.subject.otherTriskelionses_ES
dc.titleIdentifying the function of vesicle trafficking in geminiviral infection using virus induced gene silencinges_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXL Congreso de la Sociedad Española de Genéticaes_ES
dc.relation.eventplaceCórdobaes_ES
dc.relation.eventdate16-10-2015es_ES
dc.rights.ccby-nc-nd


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