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dc.contributor.authorClaros-Gil, Silvia 
dc.contributor.authorAraque-Monrós, Mª Carmen
dc.contributor.authorRodríguez-Losada, Noela 
dc.contributor.authorGil-Santos, Luis
dc.contributor.authorMonleón-Pradas, Manuel
dc.contributor.authorMás-Estellés, Jorge
dc.contributor.authorAndrades-Gómez, José Antonio 
dc.contributor.authorBecerra-Ratia, José 
dc.date.accessioned2015-10-26T11:49:14Z
dc.date.available2015-10-26T11:49:14Z
dc.date.created2015
dc.date.issued2015-10-26
dc.identifier.urihttp://hdl.handle.net/10630/10593
dc.description.abstractA new concept of a regenerative and resorbable prosthesis for tendon and ligament has been developed. The prosthesis consists of a poly-lactide acid (PLA) braid, microparticles in its interior serving as cell carriers, and a surface non-adherent coating. The aim of this study is to select the most suitable support, microparticles of poly-L-lactide (PLLA) or chitosan (CHT), for carrying the cells inside the hollow PLA braid. Microparticles of these polymers were manufactured and blended with microparticles of hyaluronic acid (HA). All of them were physically and biologically characterized. Cell viability, morphology and proliferation of human mesenchymal stem cells (hMSCs) on the different supports were evaluated and compared, revealing that PLLA microparticles were the most appropriate to be used as injectable cell-carrier. Finally, hMSCs differentiation into tenocytes was carried out on PLLA microparticles using bone morphogenetic protein-12 (BMP-12) and a mixture of transforming growth factor-β1 (TGF-β1) and insulin-like growth factor1 (IGF-1). Cell morphology was analyzed by electronic and confocal microscopy and cell differentiation was evaluated immunocytochemically for the presence of type I collagen and tenomodulin. Besides, the tenomodulin and decorin gene expression were measured by real-time quantitative polymerase chain reaction (RT-qPCR). Our results showed that the medium supplemented with BMP-12 promoted higher expression of tenomodulin and decorin, both of them differentiation markers of tenocytes. This approach might be relevant to future tissue engineering applications in reconstruction of tendon and ligament defects. Authors acknowledge support of the Spanish Instituto de Salud Carlos III through CIBERbbn and the Spanish Network on Cell Therapy (Red TerCel) initiatives.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectMateriales biomédicoses_ES
dc.subject.otherTenocyteses_ES
dc.subject.otherStem cellses_ES
dc.subject.otherTendon regenerativees_ES
dc.subject.otherBiomaterialses_ES
dc.titleTenocytic induction of stem cells from bone marrow on polymeric microparticles for a new concept of tendon regenerative prosthesises_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleSociedad Española de Terapia génica y celulares_ES
dc.relation.eventplaceSan Sebastiánes_ES
dc.relation.eventdateNoviembre 2015es_ES
dc.cclicenseby-nc-ndes_ES


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