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dc.contributor.authorNarváez Jurado, Isabel
dc.contributor.authorMercado, J.A.
dc.contributor.authorJiménez-Díaz, Rafael
dc.contributor.authorPliego-Alfaro, Fernando 
dc.date.accessioned2016-03-11T11:42:18Z
dc.date.available2016-03-11T11:42:18Z
dc.date.created2016
dc.date.issued2016-03-11
dc.identifier.urihttp://hdl.handle.net/10630/11064
dc.description.abstractIn this investigation, somatic embryogenesis in explants of adult wild olive trees, using the protocol developed by Mazri et al. (2013, Sci. Hort. 159: 88-95), was induced. Four genotypes differring in the level of resistance to the fungal pathogen Verticillium dahliae were used: Ac18, StopVert and OutVert (symptomless resistant genotypes, Jiménez-Fernández et al. 2015, Plant Pathology, in press) and Ac15 (susceptible genotype, Jiménez-Díaz, IAS-CSIC, Córdoba, personal communication). Three types of explants from actively growing cultures were used: first pair of leaves, petioles and shoot apex. Firstly, all explants were cultured on a liquid induction medium with MS mineral elements at 0.5X and 30 μM TDZ-0.5 μM NAA, for 4 days at 80rpm. Afterwards, explants were transferred to basal MS with 0.5X macroelements, for 8 weeks. Finally, calli were cultured on expression ECO medium supplemented with 0.25 μM IBA, 0.5 μM 2ip and 0.44 μM BA, for several subcultures. Cultures were incubated under darkness at 25 degrees. Embryogenic calli were observed on shoot apex (StopVert) or leaf primordia (Ac18) explants.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectOlivoses_ES
dc.subjectEmbriogénesis somáticaes_ES
dc.subject.otherOlivees_ES
dc.subject.otherOlea europaees_ES
dc.subject.otherGenetic transformationes_ES
dc.subject.otherVerticillium dahliaees_ES
dc.subject.otherSomatic embryogenesises_ES
dc.subject.otherTransgenic plantses_ES
dc.titleSomatic embryogenesis in explants of adult wild olive treeses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitlePlant Cells in Vitro: Theory and Practice. VISCEA International Conferencees_ES
dc.relation.eventplaceViena (Austria)es_ES
dc.relation.eventdateFebrero 2016es_ES
dc.cclicenseby-nc-ndes_ES


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