Before the rise of the Multidimentional Protein
Identification Technology (MudPIT), protein and
peptide mixtures were resolved using traditional
proteomic technologies like the gel-‐ based 2D chromatography that separates proteins by isoelectric point and molecular weight. This technique was tedious and limited, since the
characterization of single proteins required isolation of protein gel spots, their subsequent
proteolyzation and analysis using Matrix-‐ assisted laser desorption/ionization-‐time of
flight (MALDI-‐TOF) mass spectrometry.
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