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dc.contributor.authorLópez-Márquez, Diego
dc.contributor.authorRodríguez-Negrete, Edgar
dc.contributor.authorLópez-Pagán, Nieves
dc.contributor.authorZumaquero, Adela
dc.contributor.authorRodriguez-Bejarano, Eduardo 
dc.contributor.authorBeuzon-Lopez, Carmen del Rosario 
dc.date.accessioned2016-06-27T11:32:01Z
dc.date.available2016-06-27T11:32:01Z
dc.date.created2016
dc.date.issued2016-06-27
dc.identifier.urihttp://hdl.handle.net/10630/11666
dc.description.abstractTwo main types of noncoding small RNA molecules have been found in plants: microRNAs (miRNAs) and small interfering RNAs (siRNAs). They differ in their biogenesis and mode of action, but share similar sizes (20-24 nt). Their precursors are processed by Dicer-Like RNase III (dcl) proteins present in Arabidopsis thaliana, and in their mature form can act as negative regulators of gene expression, being involved in a vast array of plant processes, including plant development, genomic integrity or response to stress. Small-RNA mediated regulation can occurs at transcriptional level (TGS) or at post-transcriptional level (PTGS). In recent years, the role of gene silencing in the regulation of expression of genes related to plant defence responses against bacterial pathogens is becoming clearer. Comparisons carried out in our lab between the expression profiles of different mutants affected in gene silencing, and plants challenged with Pseudomonas syringae pathovar tomato DC3000, led us to identify a set of uncharacterized R genes, belonging to the TIR-NBS-LRR gene family, differentially expressed in these conditions. Through the use of bioinformatics tools, we found a miRNA* of 22 nt putatively responsible for down-regulating expression of these R genes through the generation of siRNAs. We have also found that the corresponding pri-miRNA is down-regulated after PAMP-perception in a SA-dependent manner. We also demonstrate that plants with altered levels of miRNA* (knockdown lines or overexpression lines) exhibit altered PTI-associated phenotypes, suggesting a role for this miRNA* in this defence response against bacteria. In addition we identify one of the target genes as a negative regulator of defence response against Pseudomonas syringae.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. MINECO, FEDERes_ES
dc.language.isospaes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectBacterias patógenases_ES
dc.subjectBacteriases_ES
dc.subject.otherVirulenciaes_ES
dc.subject.otherPatógenoses_ES
dc.subject.otherPlantases_ES
dc.subject.otherDefensaes_ES
dc.subject.otherResistenciaes_ES
dc.subject.otherSilenciamiento génicoes_ES
dc.titlePAMP-triggered immunity against Pseudomonas syringae involves microRNA-mediated regulation of several uncharacterized R geneses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXIII Reunión de Biología Molecular de Plantases_ES
dc.relation.eventplaceOviedoes_ES
dc.relation.eventdateJunio 2016es_ES
dc.identifier.orcidhttp://orcid.org/0000-0002-6888-3845es_ES
dc.cclicenseby-nc-ndes_ES


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