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dc.contributor.authorFernández-Arjona, María del Mar
dc.contributor.authorLópez-Ávalos, María Dolores 
dc.contributor.authorMateos-Grondona, Jesus 
dc.contributor.authorFernández-Llebrez, Pedro 
dc.date.accessioned2016-07-12T10:06:46Z
dc.date.available2016-07-12T10:06:46Z
dc.date.created2016
dc.date.issued2016-07-12
dc.identifier.urihttp://hdl.handle.net/10630/11818
dc.descriptionPoster en Congresoes_ES
dc.description.abstractSeveral studies show that morphological changes of microglia over the course of inflammation are tightly coupled to function. However the progressive transformation into activated microglia is poorly characterized. AIMS: This study aimed to establish a spatiotemporal correlation between quantifiable morphological parameters of microglia and the spread of an acute ventricular inflammatory process. METHODS: Inflammation was induced by a single injection of the enzyme neuraminidase within the lateral ventricle of rats. Animals were sacrificed 2, 4 and 12 hours after injection. Coronal slices were immunostained with Iba1 to label microglia and with IL1β to delimit the spread of inflammation. Digital images were obtained by scanning the labelled sections. Single microglia images were randomly selected from periventricular areas of caudate putamen, hippocampus and hypothalamus. FracLac for ImageJ software was used to measure the following morphological parameters: fractal dimension, lacunarity, area, perimeter and density. RESULTS: Significant differences were found in fractal dimension, lacunarity, perimeter and density of microglia cells of neuraminidase injected rats compared to sham animals. However no differences were found in the parameter “area”. In hipoccampus there was a delay in the significant change of the measured parameters. These morphological changes correlated with IL1β-expression in the same areas. CONCLUSIONS: Ventricular inflammation induced by neuraminidase provokes quantifiable morphological changes in microglia restricted to areas labelled with IL1β. Morphological parameters of microglia such as fractal dimension, lacunarity, perimeter and density are sensitive and valuable tools to quantify activation. However, the extensively used parameter “area” did not change upon microglia activation.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Grant: Junta de Andalucía (Spain) P11-CVI-07637 Ibima, Andalucía Tech, UMAes_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectInflamación (Patología)es_ES
dc.subject.otherNeuroinflamaciónes_ES
dc.subject.otherNeuraminidasaes_ES
dc.titleMorphological traits of surveillant/activated microglia during an experimentally induced acute neuroinflammatory processes_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle10 FENS Forum of Neuroscienceses_ES
dc.relation.eventplaceCopenhagen, Denmarkes_ES
dc.relation.eventdate2-6 Julio 2016es_ES
dc.identifier.orcidhttp://orcid.org/0000-0003-1409-5816es_ES
dc.cclicenseby-nc-ndes_ES


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