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dc.contributor.authorNarváez-Peláez, Manuel 
dc.contributor.authorBorroto Escuela, Dasiel Óscar
dc.contributor.authorMillón-Peñuela, Carmelo 
dc.contributor.authorFlores-Burgess, Antonio 
dc.contributor.authorGago-Calderón, Belén 
dc.contributor.authorSantín-Núñez, Luis Javier 
dc.contributor.authorFuxe, Kjell
dc.contributor.authorNarváez-Bueno, José Ángel 
dc.contributor.authorDíaz-Cabiale, Zaida 
dc.date.accessioned2016-09-26T11:48:21Z
dc.date.available2016-09-26T11:48:21Z
dc.date.created2016-09
dc.date.issued2016-09-26
dc.identifier.urihttp://hdl.handle.net/10630/12080
dc.description.abstractPreviously, we have described Galanin(GAL) and Neuropeptide Y Y1(NPYY1) interactions at behavioural, cellular and receptor levels through GALR2/NPYY1R heterodimers in the amygdala. The aim of this work was to study GAL/NPYY1R interactions in the Dentate Gyrus (DG) of the Hippocampus, using autoradiographic, in situ hybridization and in situ proximity ligation assay (PLA). Rats (n=6) were sacrificed 15 minutes or 5 hours after icv injections of GAL (3nmol) and DG sections were incubated with NPYY1R agonist [I125]-[Leu31,Pro34]PYY (25 pM) or NPYY1R-33PdATP specific probe, for autoradiography and in situ hybridization respectively. Autoradiograms were analyzed using NIH image analysis system and Student’s unpaired t-test was used. For PLA, DG sections were incubated with anti-GALR2 Rabbit (1:100) and anti-NPYY1R Goat (1:200). PLA signals were detected with PLA PLUS or MINUS probes for rabbit or goat/mouse antibodies. PLA signals were visualized by using a confocal microscope Leica TCS-SL confocal microscope (Leica). We observed that GAL significant increased the NPYY1R agonist [I125]-[Leu31,Pro34]PYY binding in the DG by 20% (p<0,05) and the NPYY1R mRNA expression in the granular layer of DG by 31% (p<0,001). Moreover, PLA-positive red clusters were found specifically in the polymorphic layer and subgranular zone of the DG. No PLA clusters were observed neither in the molecular layer of the DG nor in the corpus callosum, an area that seems to lack of GALR2 receptor. These results demonstrate a novel mechanism of interaction between GAL and NPY1R in the DG at receptor level, probably involving the formation of GALR2/NPYY1R heteroreceptor complexes. Study supported by Junta de Andalucia CVI6476.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Study supported by Junta de Andalucia CVI6476.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectNeurotransmisoreses_ES
dc.subjectNeuropéptidoses_ES
dc.subject.otherNeuropeptide Y Y1 Receptores_ES
dc.subject.otherGalanin receptor 2es_ES
dc.subject.otherHeterodimerses_ES
dc.subject.otherDepressiones_ES
dc.subject.otherDentate gyruses_ES
dc.titleGALR2/NPYY1R heterodimers interact at receptor level in the dentate gyrus of the hippocampus in ratses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Medicinaes_ES
dc.relation.eventtitleXXXVIII CONGRESO DE LA SECF - Sociedad Española de Ciencias Fisiológicases_ES
dc.relation.eventplaceZaragozaes_ES
dc.relation.eventdate13-09-2016es_ES
dc.identifier.orcidhttp://orcid.org/0000-0003-0922-4900es_ES
dc.cclicenseby-nc-ndes_ES


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