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dc.contributor.authorLabella, Alejandro
dc.contributor.authorCarrera, David
dc.contributor.authorLópez-Bueno, Alberto
dc.contributor.authorAlejo, Alí
dc.contributor.authorBorrego-Garcia, Juan Jose 
dc.contributor.authorCastro, Dolores
dc.date.accessioned2017-06-15T07:18:37Z
dc.date.available2017-06-15T07:18:37Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/10630/13890
dc.description.abstractLymphocystis disease virus (LCDV), a member of the genus Lymphocystivirus, family Iridoviridae, is the etiological agent of the lymphocystis disease (LCD), a common pathology that has been described in more than 150 different fish species worldwide. Direct sequencing of the virome of lymphocystis lesions from affected gilthead seabream (Sparus aurata) was used to obtain the complete genome sequence of a new LCDV species, named LCDV-Sa, that is the largest vertebrate iridovirus sequenced to date. This approach allowed us to assemble the full-length circular genomes of two previously unknown viruses, tentatively identified as members of the Polyomaviridae and Papillomaviridae families, and named Sparus aurata polyomavirus 1 (SaPyV1) and Sparus aurata papillomavirus 1 (SaPV1), respectively. SaPyV1 genome is a circular 7,299-bp-long DNA with a 52.1% GC content, and contains five nonoverlapping ORFs carried on opposite DNA strands in an organization reminiscent to that found in polyomaviruses. Phylogenetic analyses based on the amino acid sequence of the large T antigen and the VP1 proteins revealed that SaPyV1 clustered with the other currently available polyomavirus-like full-length genomes obtained from fish. SaPV1 genome corresponds to a circular 5,748-bp-long DNA and has a GC content of 39.5%. Although this genome is smaller than that of previously described papillomaviruses (about 8 kbp), it presents a typical papillomavirus organization, with seven ORFs carried on the same strand. Similarity searches identified distant orthologues of the early E1 and E2 proteins involved in replication and transcription, and late structural proteins L1 and L2. Furthermore, one of the others ORFs encodes a small protein (52 amino acids) that contains both a pRB binding domain (LXCXE) and a C-terminal PDZ class 2 binding motif, with are elements typically present in the longer E7 and E6 proteins found in most known papillomaviruses. The conserved genomic organization, the similarity of the main proteins, and the phylogenetical analysis, based on the amino acid sequence of the L1 protein, support that SaPV1 is the first member of the Papillomaviridae family described in fish. Epizootic surveys carried out in gilthead seabream farms in the Mediterranean area showed that LCD is frequently associated with the concurrent appearance of one or both of the new viruses, 98.3% of diseased fish being positive for SaPyV1 and/or SaPV1. In LCDV-infected asymptomatic fish, SaPyV1 and/or SaPV1 were detected in 32.1% of the animals analysed.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.publisherInstitute for Veterinary Medical Research, Budapest, Hungaryes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectEnfermedades por viruses_ES
dc.titleNovel polyomavirus and papillomavirus detected in gilthead seabream infected by lymphocystis disease viruses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle10th Internatinal Symposium on Viruses of Lower Vertebrateses_ES
dc.relation.eventplaceBudapest, Hungaryes_ES
dc.relation.eventdateJunio 2017es_ES
dc.identifier.orcidhttp://orcid.org/0000-0002-1589-3820es_ES
dc.cclicenseby-nc-ndes_ES


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