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dc.contributor.authorMerchante, Catharina
dc.contributor.authorYun, Jeonga
dc.contributor.authorValpuesta-Fernandez, Victoriano 
dc.contributor.authorStepanova, Anna
dc.contributor.authorAlonso, José
dc.date.accessioned2017-06-20T08:53:33Z
dc.date.available2017-06-20T08:53:33Z
dc.date.created2017
dc.date.issued2017-06-20
dc.identifier.urihttp://hdl.handle.net/10630/13941
dc.description.abstractTranslational regulation has long been recognized as a vital process in the cell responses to the environment and in the execution of developmental programs, yet still little is known about the selective translation of specific mRNAs and its regulation. By the implementation of the Ribo-Seq technology in Arabidopsis [1], we uncovered a translation regulation module in response to ethylene that involves the key-signalling gene EIN2, the 3´UTRs of EBF1 and 2 and the NMD machinery [2]. We now focus on the ead1,2 mutants, which present altered responses to ethylene and auxin. The two corresponding genes represent the likely orthologs of translation factors from yeast and humans, and this, together with their inferred roles in multiple response pathways, offer an excellent opportunity to investigate the role of signal integration at the translational level. EAD1 and 2 interact and Ribo-seq on ead1 revealed an accumulation of translating ribosomes in the 5´UTRs of uORF-containing genes and reduction in the levels of ribosomes in the main ORF. ead1 is also impaired in the translation of GFP when fused to WT 5´UTR of potential EAD1 targets but not when fused to uORF-less versions of the same 5´UTRs. Our hypothesis is that, under certain hormonal and/or environmental conditions, EAD1/2 work as a complex required for the efficient translation of mRNAs that have common structural and functional features. Our progress towards the identification of the conditions where the EAD1 regulation of translation is required will be presented. [1] Merchante C et al. Bio-Protocol, 6(21), 1–34. [2] Merchante C et al. (2015) Cell, 163(3): 684-697es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectRegulación genéticaes_ES
dc.subject.otherTranslation controles_ES
dc.titleTranslation regulation of uORFs-containing genes in Arabidopsises_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleAt the forefront of plant researches_ES
dc.relation.eventplaceGante, Béligcaes_ES
dc.relation.eventdate15-16 Junio 2017es_ES
dc.cclicenseby-nc-ndes_ES


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