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dc.contributor.authorRic-Varas, Pablo
dc.contributor.authorPosé, Sara
dc.contributor.authorBarceló, Marta
dc.contributor.authorKnox, Paul
dc.contributor.authorBlanco-Portales, Rosario
dc.contributor.authorMuñoz-Blanco, Juan
dc.contributor.authorMatas, Antonio J.
dc.contributor.authorQuesada, Miguel Ángel
dc.contributor.authorMercado, José A.
dc.date.accessioned2017-07-04T09:59:17Z
dc.date.available2017-07-04T09:59:17Z
dc.date.created2017
dc.date.issued2017-07-04
dc.identifier.urihttp://hdl.handle.net/10630/14100
dc.description.abstractStrawberry softening is one of the main factors that reduces fruit quality and leads to economically important losses. Textural changes during fruit ripening are mainly due to the dissolution of middle lamellae, a reduction in cell-to-cell adhesion and the weakening of parenchyma cell walls as a result of the action of cell wall modifying enzymes. Functional studies of genes encoding pectinase enzymes (polygalacturonase, pectate lyase and -galactosidase) support a key role of pectin disassembly in strawberry softening. Evidence that RG-I may play an important role in strawberry texture has been obtained from the transient silencing of a RG-lyase gene. Pectins are major components of fruit cell walls and highly dynamic polysaccharides, but due to their heterogeneity the precise relation between the structures and functions is incomplete. In this work, stable transgenic strawberry lines with a rhamnogalacturonate lyase gene (FaRGLyase1) down-regulated have been analyzed. Several transgenic lines showing more than 95% silencing of FaRGLyase1 displayed fruit firmness values higher than control. Cell walls from these lines were extracted and analyzed by ELISA and Epitope Detection Chromatography (EDC). This last technique is based on the detection of specific cell wall oligosaccharide epitopes and provides information on sub-populations of pectins containing homogalacturonan and RG-I domains, but also reveals potential links with other cell wall polysaccharides such as xyloglucan. The results obtained indicate that the silencing of FaRGLyase1 reduces degradation of RG-I backbones, but also homogalacturonan, in cell walls, especially in pectin fractions covalently bound to the cell wall. These changes contribute to the increased firmness of transgenic fruits.es_ES
dc.description.sponsorshipThis research was supported by FEDER EU Funds and the Ministerio de Economía y Competitividad of Spain (grant reference AGL2014-55784-C2), a Marie Curie IEF within the 7th European Community Framework Programme (reference: PIEF-2013-625270) for SP and a FPI fellowship (BES-2015-073616) to support PR-V. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectFresas - Fisiologíaes_ES
dc.subject.otherPlant cell walles_ES
dc.subject.otherPlant physiologyes_ES
dc.subject.otherPectines_ES
dc.subject.otherRhamnogalacturonase Lyasees_ES
dc.subject.otherStrawberryes_ES
dc.titleRhamnogalacturonase lyase gene downregulation in strawberry and its potential on mechanical fruit propertieses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXXII Meeting of the Spanish Society of Plant Physiology and the XV Hispano-Portuguese Congress of Plant Physiologyes_ES
dc.relation.eventplaceBarcelona, Spaines_ES
dc.relation.eventdate2017/06/26es_ES
dc.identifier.orcidhttp://orcid.org/0000-0003-4348-3930es_ES
dc.cclicenseby-nc-ndes_ES


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