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dc.contributor.authorVida, Carmen
dc.contributor.authorTienda, Sandra
dc.contributor.authorDe-Vicente-Moreno, Antonio 
dc.contributor.authorCazorla-Lopez, Francisco Manuel 
dc.contributor.authorGutiérrez-Barranquero, José A.
dc.date.accessioned2017-07-18T11:23:49Z
dc.date.available2017-07-18T11:23:49Z
dc.date.created2017
dc.date.issued2017-07-18
dc.identifier.urihttp://hdl.handle.net/10630/14256
dc.description.abstractBackgrounds This study focused on the characterization and selection of bacterial strains obtained from a suppressive soil displaying antifungal activity against the soilborne phytopathogenic fungi Rosellinia necatrix. Bacterial profile from this suppressive soil were first obtained by 16S rRNA gene sequencing, revealing a significant increase in the bacterial class Gammaproteobacteria, especially in some antagonistic representatives of Pseudomonas spp. Objectives To obtain and characterize a collection of 246 bacterial isolates obtained from this suppressive soil, in order to identify new strains with antifungal activity against fungal phytopathogens. Methods To obtain the bacterial collection, we performed an isolation on a selective medium for Pseudomonas-like microorganisms. Further characterization tests were used in order to analyse the bacterial collection, including identification of the general metabolic profile of glucose, the profiling of antifungals produced, including both the putative production of antifungal compounds and lytic exoenzymes, and the evaluation of traits related with beneficial effects on plants. Conclusions A final selection of representative strains resulted in antifungal isolates belonging to the genus Pseudomonas, but also some representatives of the genera Serratia and Stenotrophomonas. These selected strains were tested for plant protection by an in vivo experiment using avocado and wheat plants challenged by the pathogen R. necatrix, showing all of them an antifungal ability and plant disease protection. Pseudomonas-like strains isolated from suppressive soils constitute an excellent source for novel microbial biocontrol agents against soilborne fungal pathogens. This work was supported by grant AGL2014-52518-C2-1-R. Carmen Vida and Sandra Tienda are supported by a PhD fellowship from the FPI program of the Spanish Government.es_ES
dc.description.sponsorshipThis work was supported by grant AGL2014-52518-C2-1-R. Carmen Vida and Sandra Tienda are supported by a PhD fellowship from the FPI program of the Spanish Government; Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectHongos fitopatógenoses_ES
dc.subject.otherBiocontroles_ES
dc.subject.otherAguacatees_ES
dc.subject.otherRosellinia necatrixes_ES
dc.titleIsolation, characterization and selection of bacterial isolates from a suppressive soil with beneficial traits to plantses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle7th Congress of European Microbiologistses_ES
dc.relation.eventplaceValenciaes_ES
dc.relation.eventdate9-13 julioes_ES
dc.identifier.orcidhttp://orcid.org/0000-0003-0798-0964es_ES
dc.cclicenseby-nc-ndes_ES


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