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Evaluation of alternative IAA biosynthetic pathways in the bacteria from the pseudomonas syringae complex
dc.contributor.author | Pintado, Adrián | |
dc.contributor.author | Pérez-Martínez, Isabel | |
dc.contributor.author | Cerezo, Miguel | |
dc.contributor.author | Flors, Víctor | |
dc.contributor.author | Ramos-Rodríguez, Cayo Juan | |
dc.date.accessioned | 2017-07-27T11:20:27Z | |
dc.date.available | 2017-07-27T11:20:27Z | |
dc.date.created | 2017 | |
dc.date.issued | 2017-07-27 | |
dc.identifier.uri | http://hdl.handle.net/10630/14399 | |
dc.description.abstract | Indole-3-acetic acid (IAA) is a phytohormone belonging to the auxin group which production is widely distributed among plant-associated bacteria. In phytopathogenic bacteria, several IAA biosynthetic pathways have been described. The best characterized is the indole-3-acetamide (IAM) pathway, where tryptophan is initially converted into IAM by a monooxygenase (iaaM gene), and later transformed to IAA in a reaction catalysed by a hydrolase (iaaH gene). Pseudomonas savastanoi pv. savastanoi NCPPB 3335 (Psv), which synthesizes IAA through IAM, encodes two paralogs of these two genes organized in two operons (iaaMH-1 and iaaMH-2). Previously, we have demonstrated that a Psv mutant in the iaaMH-1 operon produces an amount of IAA significantly lower than that synthesized by the wild type strain. This strain, shows a reduced virulence in olive plants. In contrast, a mutant in the iaaMH-2 operon (which encodes a iaaM-2 pseudogene), produces IAA levels similar to those of the wild type strain and is not affected in virulence. Unexpectedly, the iaaMH-1 mutant and the double mutant iaaMH- 1/iaaMH-2 synthesize a residual amount of IAA, suggesting the existence of an alternative route for the production of this compound in Psv. | es_ES |
dc.description.sponsorship | Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. | es_ES |
dc.language.iso | eng | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Bacterias | es_ES |
dc.subject.other | Phytohormone | es_ES |
dc.subject.other | Pseudomonas savastanoi | es_ES |
dc.subject.other | IAA | es_ES |
dc.title | Evaluation of alternative IAA biosynthetic pathways in the bacteria from the pseudomonas syringae complex | es_ES |
dc.type | info:eu-repo/semantics/conferenceObject | es_ES |
dc.centro | Facultad de Ciencias | es_ES |
dc.relation.eventtitle | 7th FEMS Congress of European Microbiologists | es_ES |
dc.relation.eventplace | Valencia, España | es_ES |
dc.relation.eventdate | Julio, 2017 | es_ES |
dc.cclicense | by-nc-nd | es_ES |