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dc.contributor.authorMartín-Clemente, Elena
dc.contributor.authorMelero Jiménez, Ignacio José
dc.contributor.authorReul, Andreas 
dc.contributor.authorHernández-López, Miguel
dc.contributor.authorSalvo-Tierra, Angel Enrique 
dc.contributor.authorBañares-España, Elena 
dc.contributor.authorGarcía-Sánchez, María Jesús 
dc.contributor.authorFlores-Moya, Antonio 
dc.date.accessioned2017-09-15T07:16:58Z
dc.date.available2017-09-15T07:16:58Z
dc.date.created2017
dc.date.issued2017-09-15
dc.identifier.urihttp://hdl.handle.net/10630/14471
dc.description.abstractLos Baños de la Hedionda (Málaga, S Spain) is a natural sulphureous spa (150-200 µM sulphide). Although this high sulphide levels can affect the photosynthetic process, there are numerous photosynthetic microorganisms inhabiting the spa. Among them, we isolated a strain of the cyanobacterium Oscillatoria sp., a genus well known by its tolerance to sulphide. Objectives Firstly, to analyze the photosynthetic characteristics and growth rate of the isolated strain, as well as the effect of the presence of sulphide in both processes. Secondly, to determine the limit of genetic adaptation of this strain to sulphide. Methods The resistance of the isolated strain to sulphide was studied by analyzing the effect of increasing sulphide levels (up to 1600 µM) on photosynthetic performance and growth. The limit of genetic adaptation was explored using an evolutionary experimental design named as ratchet protocol. This design allows discerning the maximum capacity of genetic adaptation of Oscillatoria sp. to the exposure of increasing doses of sulphide Conclusions The strain showed maximum growth rates at 200 µM sulphide although reduced rates can be found up to 800 µM sulphide. A significant increase in resistance was achieved in all derived populations during the ratchet experiment (surviving at sulphide concentrations higher than 2 mM). Moreover, they showed different evolutionary potential to adapt to sulphide, depending on historical contingency.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.Spanish Ministry of Science and Innovation through CGL2014-53682-P project. Predoctoral State Grant from Scientific and Technical Research and Innovation Plan, Spanish Ministry of Economy, Industry and Competitiveness I+D+i ECC/1402/2013, 2015es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectCianobacteriases_ES
dc.subject.otherOscillatoria sp.es_ES
dc.subject.otherSulphidees_ES
dc.subject.otherRatchet protocoles_ES
dc.subject.otherAdaptationes_ES
dc.titleCharacterization of the cyanobacterium Oscillatoria sp. isolated from extreme sulphureous water from Los Baños de la Hedionda (S Spain)es_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleFEMS 7th Congress of European Microbiologistses_ES
dc.relation.eventplaceValencia, Spaines_ES
dc.relation.eventdateJulio, 2017es_ES
dc.cclicenseby-nc-ndes_ES


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