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dc.contributor.authorMartín-Pizarro, Carmen
dc.contributor.authorPosé, David
dc.date.accessioned2017-09-20T11:40:22Z
dc.date.available2017-09-20T11:40:22Z
dc.date.created2017
dc.date.issued2017-09-20
dc.identifier.urihttp://hdl.handle.net/10630/14491
dc.description.abstractDue to its octoploid nature, gene functional analyses in the cultivated strawberry (Fragaria × ananassa) are commonly carried out via gene silencing using self-complementary “hairpin” double-stranded RNA (RNAi) constructs. However, this system is not always as efficient as expected. First, an efficient silencing of the target gene is not always achieved, and second, its effect might not be stable after several clonal propagations of the transgenic lines. Recently, genome editing is becoming an important biotechnological tool for gene functional analysis and crop improvement, in particular since the development of the CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat-CRISPR associated protein 9) system. To investigate the functionality of the CRISPR/Cas9 in strawberry, we designed two sgRNAs directed against two regions of the floral homeotic gene APETALA3 (AP3) in order to induce a deletion of around 200 nt. A vector containing both sgRNAs and Cas9 was used to transform leaf disks of F. × ananassa cv. Camarosa. Several independent stable transgenic lines displayed defects in stamen and fruit development, partially phenocopying that of the Arabidopsis ap3 mutants. Molecular analysis of the targeted AP3 locus indicated differences in gene editing among different transgenic lines and suggests mutations in all the possible AP3 alleles. Phenotypic analyses indicate that impaired fruit development might be caused by the lack of proper development of the anthers due to the CRISPR/Cas9 induced mutation in AP3. In summary, we show that the CRISPR/Cas9 system is a functional tool to perform genome editing in the octoploid F. × ananassa. We propose this system as an alternative to the traditional RNAi strategy to stably mutagenize a particular gene of interest for functional analyses in this species.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectFresases_ES
dc.subject.otherStrawberryes_ES
dc.subject.otherCRISPR/Cas9es_ES
dc.titleGenome Editing of the Octoploid Fragaria x ananassa Using the CRISPR/Cas9 Systemes_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXII Reunión de la Sociedad Española de Cultivo In Vitro de Tejidos Vegetaleses_ES
dc.relation.eventplaceMadrides_ES
dc.relation.eventdate13 septiembre 2017es_ES
dc.identifier.orcidhttp://orcid.org/0000-0003-3332-4661es_ES
dc.cclicenseby-nc-ndes_ES


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