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dc.contributor.authorCaballo-Ponce, Eloy
dc.contributor.authorWittich, Regina Michaela
dc.contributor.authorvan Dillewijn, Pieter
dc.contributor.authorRamos, Cayo
dc.date.accessioned2017-09-26T12:20:56Z
dc.date.available2017-09-26T12:20:56Z
dc.date.created2017
dc.date.issued2017-09-26
dc.identifier.urihttp://hdl.handle.net/10630/14539
dc.description.abstractMost strains of the Pseudomonas syringae complex isolated from the trunks of woody plants and belonging to phylogroups (PGs) 1 and 3 share a genomic region, named WHOP (from woody host and Pseudomonas), which is partially conserved in some PG2 strains. Annotation of the 14 ORFs encoded within this region, which is absent in the genomes of P. syringae strains infecting herbaceous hosts, yielded functions possibly involved in the metabolism of phenolic compounds. The genetic organization of this region was analyzed by RT-PCR in Pseudomonas savastanoi pv. savastanoi (Psv), the causative agent of olive knot disease. The WHOP region is organized in four operons (antABC, catBCA, ipoABC and dhoAB) and three genes transcribed independently (antR, benR and PSA3335_3206). HPLC analyses confirmed that the antABC and catBCA operons mediate the catabolism of anthranilate and catechol, respectively, through the β-ketoadipate pathway. In addition, oxygenase activity on aromatic compounds, tested as the conversion of indole into indigo, was assigned to the ipoABC operon. Pathogenicity tests revealed that deletion of antABC, catBCA or ipoABC in Psv caused reduced symptoms in woody olive plants, while such phenotype was not observed in young micropropagated (non-woody) plants. Similarly, the catBCA and dhoAB operons and the PSA3335_3206 gene (encoding a putative aerotaxis receptor) are also required for full bacterial fitness exclusively in woody olive plants. At present, we are addressing the role in virulence of the orphan benR gene encoded in the WHOP region.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectPseudomonases_ES
dc.subject.otherPseudomonas savastanoies_ES
dc.subject.otherVirulencees_ES
dc.subject.otherWoody hostes_ES
dc.subject.otherAdaptationes_ES
dc.subject.otherPlant pathogenesises_ES
dc.titleContribution of the metabolism of phenolic compounds to the virulence of Pseudomonas savastanoi in woody hostses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle16th International Conference on Pseudomonases_ES
dc.relation.eventplaceLiverpool (Reino unido)es_ES
dc.relation.eventdate5 de septiembre de 2017es_ES
dc.identifier.orcidhttp://orcid.org/0000-0003-0501-3321es_ES
dc.cclicenseby-nc-ndes_ES


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