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dc.contributor.authorGutiérrez-Barranquero, José A.
dc.contributor.authorCazorla-Lopez, Francisco Manuel 
dc.contributor.authorDe-Vicente-Moreno, Antonio 
dc.contributor.authorSundin, George W.
dc.date.accessioned2017-10-03T09:59:06Z
dc.date.available2017-10-03T09:59:06Z
dc.date.created2017
dc.date.issued2017
dc.identifier.citationGutierrez-Barranquero et al., 2017. Genomic analysis of eight native plasmids of the phytopathogen Pseudomonas syringae. Proceedings of the 16th International Conference on Pseudomonas. 05-09 September 2017. Liverpool. UK.es_ES
dc.identifier.urihttp://hdl.handle.net/10630/14573
dc.descriptionComunicación a conferenciaes_ES
dc.description.abstractThe pPT23A family of plasmids (PFPs) appears to be indigenous to the plant pathogen Pseudomonas syringae and these plasmids are widely distributed and widely transferred among pathovars of P. syringae and related species. PFPs are sources of accessory genes for their hosts that can include genes important for virulence and epiphytic colonization of plant leaf surfaces. Further understanding of the evolution of the pPT23A plasmid family and the role of these plasmids in P. syringae biology and pathogenesis, requires the determination and analysis of additional complete, closed plasmid genome sequences. Therefore, our main objective was to obtain complete genome sequences from PFPs from three different P. syringae pathovars and perform a comparative genomic analysis. In this work plasmid DNA isolation, purification by CsCl-EtBr gradients, and sequencing using 454 platform, were used to obtain the complete sequence of P. syringae plasmids. Different bioinformatic tools were used to analyze the plasmid synteny, to identify virulence genes (i.e. type 3 effectors) and to unravel the evolutionary history of PFPs. Our sequence analysis revealed that PFPs from P. syringae encode suites of accessory genes that are selected at different levels (universal, interpathovar and intrapathovar). The conservation of type IVSS encoding conjugation functions also contributes to the distribution of these plasmids within P. syringae populations. Thus, this study contributes to unravel the genetic basis of the role of PFPs in different P. syringae lifestyles.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectGenomases_ES
dc.subject.otherPlasmidoses_ES
dc.subject.otherMangoes_ES
dc.subject.otherPseudomonas syringaees_ES
dc.subject.otherGenómicaes_ES
dc.titleGenomic analysis of eight native plasmids of the phytopathogen Pseudomonas syringaees_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle16th international conference on Pseudomonases_ES
dc.relation.eventplaceLiverpool, Reino Unidoes_ES
dc.relation.eventdate05 september 2017es_ES
dc.identifier.orcidhttp://orcid.org/0000-0003-0798-0964es_ES
dc.cclicenseby-nc-ndes_ES


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