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Effects of Nanocrystalline Glass-like Carbon thin films on the SN4741 Dopaminergic cell Parkinson model
dc.contributor.author | Rodriguez-Losada | |
dc.contributor.author | Romero, Pablo | |
dc.contributor.author | Estivill-Torrús, Guillermo | |
dc.contributor.author | Guzmán de Villoria, Roberto | |
dc.contributor.author | Aguirre-Gómez, José Ángel | |
dc.date.accessioned | 2017-10-04T12:23:19Z | |
dc.date.available | 2017-10-04T12:23:19Z | |
dc.date.created | 2014 | |
dc.date.issued | 2017-10-04 | |
dc.identifier.uri | http://hdl.handle.net/10630/14581 | |
dc.description | abstract de congreso | es_ES |
dc.description.abstract | The interest in carbon nanomaterials has grown within the last decade in view of a wide variety of applications. In this work we test the biocompatibility of a particular nanometer-thin nanocrystalline glass-like carbon films (NGLC), a disordered structure of graphene flakes joined by carbon matrix [1]. We used a cell line (SN4741) from substantia nigra dopaminergic cells derived from transgenic mouse embryo cells [2]. Some cells were cultured on top of NGLC films (5, 20 and 80 nm) and other with NGLC microflakes (approx. 5-10 mm2) in increasing concentrations: 1, 5, 10, 20 and 50 μg/ml, during 24 h, 3 days and 7 days. Cells growing in normal conditions were defined under culture with DMEM supplemented with 10% FCS. Microflakes were resuspended in DMEM at the stock concentration (2 g/l). We use 96 well plates (Corning) using 2500 cells per well. For MTT analysis a positive control with a 10% Triton X-100 and a negative control. As apoptosis/necrosis assay we used LIVE/DEAD® Viability/Cytotoxicity Assay Kit (Invitrogen). In a separate experiment, cells were cultured on top of the NGLC films for 7 days. Primary antibodies: anti-synaptophysin (SYP, clone SY38, Chemicon) and goat anti-GIRK2 (G-protein-regulated inward-rectifier potassium channel 2 protein) (Abcom) for immunofluorescence. WB was performed with a polyclonal anti-rabbit proliferating cell nuclear antigen (PCNA). We demonstrate the biocompatibility with different concentration of NGLC varying the degree of survival from a low concentration (1 g/ml) in the first 24 h to high concentrations (20-50 g/ml) after 7 days, corroborated by the PCNA analysis. Cells cultured on top of the film showed after 7 days axonal-like alignment and edge orientation as well as net-like images. Neuronal functionality was demonstrated through the analysis of coexistence between SYP and GIRK2. This nanomaterial could offer a powerful platform for biomedical applications such as neural tissue engineering. | es_ES |
dc.description.sponsorship | Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. | es_ES |
dc.language.iso | spa | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Carbono | es_ES |
dc.subject.other | Nanocrystalline glass-like carbon | es_ES |
dc.subject.other | Graphene related materials | es_ES |
dc.subject.other | SN4741 cells | es_ES |
dc.subject.other | Parkinson's model | es_ES |
dc.title | Effects of Nanocrystalline Glass-like Carbon thin films on the SN4741 Dopaminergic cell Parkinson model | es_ES |
dc.type | info:eu-repo/semantics/conferenceObject | es_ES |
dc.centro | Facultad de Medicina | es_ES |
dc.relation.eventtitle | 17th National Congress of the Spanish Society of Neuroscience | es_ES |
dc.relation.eventplace | Alicante | es_ES |
dc.relation.eventdate | 27 Septiembre 2017 | es_ES |
dc.identifier.orcid | http://orcid.org/0000-0003-1305-4123 | es_ES |
dc.rights.cc | by-nc-nd |