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dc.contributor.authorRico-Llanos, Gustavo
dc.contributor.authorBecerra, José
dc.contributor.authorVisser, Rick
dc.date.accessioned2017-11-24T10:31:02Z
dc.date.available2017-11-24T10:31:02Z
dc.date.created2017
dc.date.issued2017-11-24
dc.identifier.urihttp://hdl.handle.net/10630/14836
dc.description.abstractBone morphogenetic protein-2 (BMP-2) is widely used in orthopedic surgery and bone tissue engineering because of its strong osteogenic activity. However, BMP-2 treatments have several drawbacks and alternatives are being explored. Since BMP-6 has been demonstrated to be more osteoinductive, its use, either alone or together with other cytokines, might be an interesting option. We have compared the effect of BMP-2, BMP-6, or insulin-like growth factor-1 (IGF-1), either alone or in combination. MC3T3-E1 cells were treated with IGF-1 and/or of BMP-2 or -6 and the expression of osteogenic genes, proliferation and alkaline phosphatase (ALP) activity in vitro were analyzed. The results showed that IGF-1 greatly enhanced the BMP-induced osteogenic differentiation of these cells and that the ALP activity in the cultures was higher when the combination was made with BMP-6 than with BMP-2. Other in vitro experiments showed that the osteogenic effect of these combinations can be modulated controlling the sequential administration of the growth factors. Furthermore, we have tested the osteogenic potential of these treatments in vivo by loading them onto absorbable collagen sponges which were implanted into an ectopic bone formation model in rats. These experiments revealed that only BMP-6 was able to induce bone formation at the used dose and that the addition of IGF-1 contributed to an increase of the mineralization in the implants. Hence, the combination of BMP-6 with IGF-1 might be a better alternative than BMP-2 for orthopedic surgery and bone tissue engineering approaches with potential application through using controlled delivery systems.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectIngeniería de tejidoses_ES
dc.subject.otherBonees_ES
dc.subject.otherTissue engineeringes_ES
dc.subject.otherBone morphogenetic proteinses_ES
dc.subject.otherInsulin-like growth factores_ES
dc.subject.otherCollagenes_ES
dc.titleIGF-1 enhances the osteogenic activity of BMP-6 in vitro and in vivo, and together have a stronger osteogenic effect than when IGF-1 is combined with BMP-2es_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleJoint Congress 2017es_ES
dc.relation.eventplaceGijón, Españaes_ES
dc.relation.eventdate24/10/2017es_ES
dc.cclicenseby-nc-ndes_ES


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