Mostrar el registro sencillo del ítem

dc.contributor.authorLópez-Unzu, Miguel A.
dc.contributor.authorDurán-Boyero, Ana Carmen 
dc.contributor.authorSoto-Navarrete, María Teresa
dc.contributor.authorFernández-Corujo, Borja 
dc.date.accessioned2018-05-21T07:29:14Z
dc.date.available2018-05-21T07:29:14Z
dc.date.created2018
dc.date.issued2018-05-21
dc.identifier.urihttps://hdl.handle.net/10630/15766
dc.description.abstractIn extant vertebrates, myosin heavy chain (MyHC) 6 and 7 are the main isoforms of atrial and ventricular myocardium respectively, whereas MyHC7b has been proposed to be an ancient cardiac isoform only expressed during embryonic development in modern species. In preliminary immunohistochemical studies of the heart of the lesser spotted dogfish (Scyliorhinus canicula; Chondrichthyes), we have observed that while MF20 labels homogeneously all the myocardium, A4.1025 labels the inflow cardiac segments (sinus venosus and atrium) but not the outflow segments (ventricle and conus arteriosus). In order to interpret these results, we have performed western and slot blots from samples of dogfish and hamster (Mesocricetus auratus) hearts, HPLC-ESI-MS/MS from dogfish samples, and immunohistochemistry in hearts of representative species of vertebrates, namely elasmobranchs, polypteriforms, acipenseriforms, teleosts and mammals, using MF20 and A4.1025 antibodies. Western and slot blot results confirmed the specificity of MF20 and A4.1025 for MyHC in dogfish, as well as their differential reactivity against different myocardial segments. HPLC-ESI-MS/MS using protein databases from Callorhinchus milii (Chondrichthyes) and Chordata revealed the presence of MyHC6 and 7 in all the dogfish myocardial segments, and of MyHC7b only in the outflow segments. Immunohistochemistry showed that while MF20 signals were homogeneous in all the myocardial segments of all the species studied, A4.1025 signals were restricted to the inflow myocardial segments in elasmobranchs, homogeneous in teleosts and acipenseriforms, and low in the ventricle of polypteriforms. It can be inferred that the A4.1025 antibody, as opposed to MF20, has a low affinity for MyHC7b, at least in the dogfish. In addition, we show that MyHC distribution in the cardiac chambers has changed during the evolution of gnathostomes.en_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.Study supported by grants CGL2017-85090-P and CGL2014-52356-P (Ministerio de Economía y Competitividad), FPU15/03209 (Ministerio de Educación, Cultura y Deporte), contract UMAJI75 (Junta de Andalucía, European Social Found), FEDER and Universidad de Málaga.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectVertebrados - Corazónen_US
dc.subjectMiosinaen_US
dc.subject.otherMyosin heavy chainen_US
dc.subject.otherHearten_US
dc.subject.otherVertebratesen_US
dc.titleChamber specific expression of Myosin heavy chain 7b in the heart of vertebratesen_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitleExperimental Biology 2018en_US
dc.relation.eventplaceSan Diego, CA, EEUUen_US
dc.relation.eventdate21 - 25 abril 2018en_US
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*


Ficheros en el ítem

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional