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dc.contributor.authorOcaña, Mª Carmen
dc.contributor.authorCarrillo, Paloma
dc.contributor.authorMartínez-Poveda, Beatriz
dc.contributor.authorQuesada, Ana R.
dc.contributor.authorMedina, Miguel Ángel 
dc.date.accessioned2018-07-17T07:28:37Z
dc.date.available2018-07-17T07:28:37Z
dc.date.created2018-07
dc.date.issued2018-07-17
dc.identifier.urihttps://hdl.handle.net/10630/16286
dc.description.abstractOne of the most studied tumor cells lines in vitro is the breast carcinoma MDA-MB-231 cell line. Several studies have proved its glycolytic profile, namely known as the Warburg effect. Glutamine oxidation is also important for its metabolism. Nevertheless, the use of fatty acids for obtaining energy in these cells is still rising. Palmitic acid is the most common saturated fatty acid, containing sixteen carbons in its structure. However, the use of palmitate for metabolic studies in MDA-MB-231 is not very extended due to its pro-apoptotic effect in this cell line after certain time exposure. Nonetheless, in this work we used palmitate as a metabolic fuel for just 30 minutes in order to see the almost immediate response of the cells to its presence, after a 30 minutes fast period. Our results show that MDA-MB-231 cells are not able of oxidizing palmitate nor producing lactate from it. Simultaneous presence of palmitate with glucose or with glutamine does not affect glucose nor glutamine uptake in these cells. However, we observed that even low concentrations of glucose increase palmitate uptake in MDA-MB-231 after a 30 minutes incubation. Treatment with 5 mM 2-deoxyglucose also for 30 minutes counters this rise, since 2-deoxyglucose diminishes palmitate uptake. Increasing glucose concentration to the same dosis of 2-deoxyglucose leads to a prevalence of the glucose effect on palmitate uptake. The exact role of glucose and glucose derivatives should be further studied in order to know more about palmitate metabolism in this cell line.en_US
dc.description.sponsorshipOur experimental work is supported by grants BIO2014-56092-R (MINECO and FEDER) and P12-CTS-1507 (Andalusian Government and FEDER) and funds from group BIO-267 (Andalusian Government). The "CIBER de Enfermedades Raras" is an initiative from the ISCIII (Spain). This communication has the support of a travel grant "Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech".en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCancer - Tratamiento - Investigación - Congresosen_US
dc.subject.otherMetabolic reprogramingen_US
dc.subject.otherBreat carcinomaen_US
dc.subject.otherGlucoseen_US
dc.subject.otherPalmitateen_US
dc.subject.otherMDA-MB-231 cellsen_US
dc.titleInterplay between glucose and palmitate uptake in breast carcinoma in vitroen_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitleThe 43rd FEBS Congressen_US
dc.relation.eventplacePraga (República Checa)en_US
dc.relation.eventdate7-12 de julio de 2018en_US
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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