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dc.contributor.authorCañas-Pendón, Rafael Antonio 
dc.contributor.authorOrtigosa, Francisco
dc.contributor.authorValderrama-Martín, José Miguel
dc.contributor.authorÁvila-Sáez, Concepción 
dc.contributor.authorCánovas-Ramos, Francisco Miguel 
dc.date.accessioned2018-07-27T09:42:01Z
dc.date.available2018-07-27T09:42:01Z
dc.date.issued2018-07-27
dc.identifier.urihttps://hdl.handle.net/10630/16371
dc.description.abstractConifers are tree species with enormous environmental and economic interests but with several characteristics that complicate their investigation (big size, secondary compounds, large long-life cycles, megagenomes…). However, they are well adapted to ammonium-rich soils being a good model to study ammonium assimilation in plants. Although they have a special feature, only two glutamine synthetase (GS, EC 6.3.1.2) genes, GS1a and GS1b, coding for cytosolic proteins, have been identified. In angiosperms and in the gymnosperm Ginkgo biloba there are two types of this enzyme responsible of the ammonium assimilation: GS1 expressed in the cytosol and GS2 in the plastids. Until the date, the searches of new GS1 and GS2 genes in conifers have been made with classical biochemical and molecular biology techniques without satisfactory results. In the present context, the emergence of the next generation sequencing (NGS) techniques has open new opportunities in the resolution of old problems. They have allowed the whole sequencing of the massive conifer genomes and the analysis of their transcriptomes. Thus, in the framework of the European project ProCoGen, a gene expression atlas of the tissues of one-month seedlings was carried out using laser capture microdissection (LCM) and massive sequencing in maritime pine (Pinus pinaster), which is a conifer tree from the Southwestern Mediterranean region1. From the analysis of this work, a new gene coding for a new putative cytosolic GS has been identified, PpGS1c. 1Cañas, RA et al. (2017). Plant J, 91. 1064-1087en_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Project funding by Ministerio de Economía y Competitividad BIO2015-69285-R and MicroNUpE (BIO2015-73512-JIN; MINECO/AEI/FEDER, UE)en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectConíferasen_US
dc.subject.otherNitrogenen_US
dc.subject.otherConiferen_US
dc.subject.otherTranscriptomicsen_US
dc.subject.otherAmmoniumen_US
dc.titleAn unexpected actor in ammonium assimilation in conifer treesen_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitleINNOVATING PLANT NUTRITION. XVII Simpósio Luso-Espanhol de Nutrição Mineral das Plantasen_US
dc.relation.eventplaceLisboa (Portugal)en_US
dc.relation.eventdate25 de julio de 2018en_US


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