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dc.contributor.authorPliego, Clara
dc.contributor.authorJiménez-Díaz, Rafael M.
dc.contributor.authorTrapero-Casas, Jose L.
dc.contributor.authorLópez-Herrera, Carlos
dc.contributor.authorArjona-López, Jose M.
dc.contributor.authorMercado, Jose A.
dc.contributor.authorFresata, Louis
dc.contributor.authorNarvaez, Isabel
dc.contributor.authorPliego-Alfaro, Fernando 
dc.date.accessioned2018-10-29T12:33:09Z
dc.date.available2018-10-29T12:33:09Z
dc.date.created2018
dc.date.issued2018-10-29
dc.identifier.urihttps://hdl.handle.net/10630/16743
dc.description.abstractThe NPR1 gene encodes a key component of SAR signaling mediated by salicylic acid (SA). After a pathogen infection, the accumulation of SA releases NPR1 monomers in the cytosol that are translocated to the nucleus, activating the expression of pathogenesis-related (PR) genes. Overexpression of NPR1 has conferred resistance to fungal, viral and bacterial pathogens in several plant species. The aim of this research was to generate transgenic olive plants expressing the gene AtNPR1 from Arabidopsis thaliana to obtain material resistant to fungal pathogens. Three transgenic lines expressing AtNPR1 gene under the control of the constitutive promoter CaMV35S were obtained following the protocol of Torreblanca et al. (2010), using an embryogenic line derived from a seed of cv. Picual. Level of AtNPR1 expression in transgenic calli varied greatly among the different lines, being higher in the line NPR1-780. The elicitation of embryogenic calli in liquid medium with AS did not increase endochitinase activity, a PR protein. However, jasmonic acid induced a transient increase in chitinase activity after 24 h of treatment in all the lines, being the increment higher in transgenic NPR1 than in control. After maturation and germination of transgenic somatic embryos, plants were micropropagated and acclimated to ex vitro conditions. The expression of AtNPR1 did not alter the growth of transgenic plants neither in vitro nor in the greenhouse. Experiments are in progress to determine the resistance of transgenic AtNPR1 plants to V. dalihae and R. necatrix.en_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Research projects: Plan Nacional AGL2014-52518-C2-1-R; AGL2017-83368-C2-1-R and Junta de Andalucía P11-AGR7992en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPlantas transgénicasen_US
dc.subjectOlivosen_US
dc.subject.otherVerticilliumen_US
dc.subject.otherGenetic transformationen_US
dc.subject.otherNPR1en_US
dc.subject.otherSomatic embryogenesisen_US
dc.subject.otherOliveen_US
dc.titleHeterologous expression of AtNPR1 gene in olive for increasing fungal toleranceen_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitleOlive Bioteqen_US
dc.relation.eventplaceSevilla, Españaen_US
dc.relation.eventdate15-19 octubre 2018en_US
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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