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dc.contributor.authorLuna, Ana P.
dc.contributor.authorRodríguez-Negrete, Edgar A.
dc.contributor.authorMorilla, Gabriel
dc.contributor.authorCastillo, Araceli G.
dc.contributor.authorBejarano, Eduardo R.
dc.date.accessioned2018-10-29T13:14:43Z
dc.date.available2018-10-29T13:14:43Z
dc.date.created2018
dc.date.issued2018-10-29
dc.identifier.urihttps://hdl.handle.net/10630/16754
dc.description.abstractGeminiviruses constitute a group of plant viruses that infect vegetable crops all over the world. Among the Geminiviridae family, the genera Mastrevirus, Begomovirus and Curtovirus are the most abundant. Suppression of gene silencing is a key mechanism for viral infection in plants. In begomovirus, V2 is a strong posttranscriptional gene silencing suppressor. We recently showed that, despite the lack of sequence homology with V2 from begomovirus, V2 from curtovirus Beet curly top virus (BCTV) is also a PTGS suppressor by impairing the RDR6/SGS3 pathway. In order to identify the domains involved the suppression activity and viral pathogenicity, we analysed the aminoacidic sequence, performing an alignment of several begomovirus and curtovirus V2 proteins. A protein kinase C (PKC) phosphorylation motif essential for suppression activity in begomovirus (P1) was founded in a similar position in all analysed sequences. Besides, other two putative CK2 and PKC phosphorilation motifs (P2 and P3) were also predicted only in BCTV V2. We also found similar hydrophobic profiles, consisting of two hydrophobic domains (H1 and H2) followed by a long hydrophilic domain. Then we generated BCTV V2 mutant proteins in each of them and performed transient assays in Nicotiana benthamiana plants to test their suppression activity. We also expressed them from a Potato virus X-derived vector to check the symptoms produced. Additionally, their subcellular localization was determined. Finally, we produced BCTV viruses mutated in the different domains and N. benthamiana plants were infected, analysing virus levels and symptoms produced. The results showed that P1, H1 and H2 are involved in the suppression activity and viral pathogenicity.en_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Techen_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTumores en plantasen_US
dc.subject.otherPTGSen_US
dc.subject.otherBCTVen_US
dc.subject.otherSilencing suppressoren_US
dc.subject.otherGeminivirusen_US
dc.titleStudy of the functional domains of V2 from Beet curly top virus (BCTV)en_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitleXIX Congreso de la Sociedad española de Fitopatologíaen_US
dc.relation.eventplaceToledoen_US
dc.relation.eventdateoctubre 2018en_US


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