JavaScript is disabled for your browser. Some features of this site may not work without it.

    Listar

    Todo RIUMAComunidades & ColeccionesPor fecha de publicaciónAutoresTítulosMateriasTipo de publicaciónCentrosEsta colecciónPor fecha de publicaciónAutoresTítulosMateriasTipo de publicaciónCentros

    Mi cuenta

    AccederRegistro

    Estadísticas

    Ver Estadísticas de uso

    DE INTERÉS

    Datos de investigaciónReglamento de ciencia abierta de la UMAPolítica de RIUMAPolitica de datos de investigación en RIUMASHERPA/RoMEODulcinea
    Preguntas frecuentesManual de usoDerechos de autorContacto/Sugerencias
    Ver ítem 
    •   RIUMA Principal
    • Investigación
    • Biología Celular, Genética y Fisiología - (BCGF)
    • BCGF - Contribuciones a congresos científicos
    • Ver ítem
    •   RIUMA Principal
    • Investigación
    • Biología Celular, Genética y Fisiología - (BCGF)
    • BCGF - Contribuciones a congresos científicos
    • Ver ítem

    Global analysis of the geminivirus tomato yellow leaf curl virus (TYLCV) genome expression and DNA methylation during infection in tomato plants

    • Autor
      Piedra-Aguilera, Álvaro; Jiao, Chen; Romero-Rodríguez, Beatriz; Esteve-Codina, Anna; Villanueva-Montiel, Francisco; Diaz-Pendón, Juan Antonio; Fei, Zhangjun; Perez-Luna, Ana IsabelAutoridad Universidad de Málaga; Rodriguez-Bejarano, EduardoAutoridad Universidad de Málaga; Castillo-Garriga, AraceliAutoridad Universidad de Málaga
    • Fecha
      2018-10-30
    • Palabras clave
      Virus
    • Resumen
      Geminiviruses are the plant largest family of DNA viruses that cause devastating diseases in crops worldwide. Upon the infection, viral genomes and transcripts are targeted by the plant gene silencing machinery, that processes the viral transcripts into viral small interfering RNAs (vsRNAs) and directs viral DNA methylation. To counteract these plant responses, geminiviruses encode proteins that are able to supress both, post-transcriptional and transcriptional gene silencing. To get an insight into geminiviral infection, the transcriptional and DNA methylation landscape of the geminivirus TYLCV (Tomato yellow leaf curl virus) from tomato-infected leaves, was characterized for the first time at high coverage by Illumina high-throughput sequencing during a time course infection. Three week-old tomato plants (Solanum lycopersicum cv. Moneymaker) were infected with TYLCV by agroinoculation or using its natural vector, the whitefly Bemisia tabaci. RNA and DNA were extracted from samples collected at 2, 7, 14 and 21 dpi (days post ingfection) and RNAseq, smallRNAseq and WGBS (Whole Genome Bisulfite Sequencing) was performed. We will present the RNA and vsRNA expression profiles along the infection and the complete DNA methylation landscape of a geminivirus by both inoculation methods, discussing the correlation with viral transcripts, vsRNA and viral DNA accumulation.
    • URI
      https://hdl.handle.net/10630/16762
    • Compartir
      RefworksMendeley
    Mostrar el registro completo del ítem
    Ficheros
    SEF201800-O49.pdf (88.81Kb)
    Colecciones
    • BCGF - Contribuciones a congresos científicos

    Estadísticas

    Ver Estadísticas de uso
    Buscar en Dimension
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
     

     

    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA