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dc.contributor.authorMartín-Pizarro, Carmen
dc.contributor.authorPosé, David
dc.date.accessioned2019-01-31T10:40:39Z
dc.date.available2019-01-31T10:40:39Z
dc.date.created2019
dc.date.issued2019-01-31
dc.identifier.urihttps://hdl.handle.net/10630/17228
dc.description.abstractThe B-class of MADS-box transcription factors has been studied in many plant species, but remain functionally uncharacterized in Rosaceae. APETALA3 (AP3), a member of this class, controls petal and stamen identities in Arabidopsis. In this study, we identified two members of the AP3 lineage in the cultivated strawberry (Fragaria x ananassa): FaAP3 and FaTM6. Interestingly, FaTM6, and not FaAP3, shows an expression pattern equivalent to that of AP3 in Arabidopsis. Genome editing using CRISPR/Cas9 system is becoming a robust tool for targeted and stable DNA mutagenesis. However, whether it can be efficiently used in an octoploid species such as F. x ananassa is not yet established. Here we report the application of CRISPR/Cas9 to characterize the function of FaTM6 in strawberry flower development. An analysis by high-throughput sequencing of the FaTM6 locus spanning the target sites showed a highly efficient genome editing already in the T0 generation. The phenotypic characterization of the mutant lines indicates that FaTM6 plays a key role in anther development in strawberry. Our results validate the CRISPR/Cas9 strategy for gene functional analysis in F. x ananassa as an octoploid species, and they offer new opportunities for engineering strawberry to improve traits of interest in breeding programs.en_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectFresasen_US
dc.subject.otherStrawberryen_US
dc.subject.otherGenome Editingen_US
dc.subject.otherCRISPR/Cas9en_US
dc.subject.otherFloweren_US
dc.subject.otherStamenen_US
dc.subject.otherTM6en_US
dc.subject.otherAP3en_US
dc.subject.otherHomeotic geneen_US
dc.titleFunctional analysis of TM6 MADS-Box gene in the octoploid strawberry by CRISPR/Cas9 directed mutagenesisen_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitlePlant & Animal Genome XXVIIen_US
dc.relation.eventplaceSan Diego, EEUUen_US
dc.relation.eventdateEnero 2019en_US
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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