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Functional analysis of TM6 MADS-Box gene in the octoploid strawberry by CRISPR/Cas9 directed mutagenesis
dc.contributor.author | Martín-Pizarro, Carmen | |
dc.contributor.author | Posé-Padilla, David | |
dc.date.accessioned | 2019-01-31T10:40:39Z | |
dc.date.available | 2019-01-31T10:40:39Z | |
dc.date.created | 2019 | |
dc.date.issued | 2019-01-31 | |
dc.identifier.uri | https://hdl.handle.net/10630/17228 | |
dc.description.abstract | The B-class of MADS-box transcription factors has been studied in many plant species, but remain functionally uncharacterized in Rosaceae. APETALA3 (AP3), a member of this class, controls petal and stamen identities in Arabidopsis. In this study, we identified two members of the AP3 lineage in the cultivated strawberry (Fragaria x ananassa): FaAP3 and FaTM6. Interestingly, FaTM6, and not FaAP3, shows an expression pattern equivalent to that of AP3 in Arabidopsis. Genome editing using CRISPR/Cas9 system is becoming a robust tool for targeted and stable DNA mutagenesis. However, whether it can be efficiently used in an octoploid species such as F. x ananassa is not yet established. Here we report the application of CRISPR/Cas9 to characterize the function of FaTM6 in strawberry flower development. An analysis by high-throughput sequencing of the FaTM6 locus spanning the target sites showed a highly efficient genome editing already in the T0 generation. The phenotypic characterization of the mutant lines indicates that FaTM6 plays a key role in anther development in strawberry. Our results validate the CRISPR/Cas9 strategy for gene functional analysis in F. x ananassa as an octoploid species, and they offer new opportunities for engineering strawberry to improve traits of interest in breeding programs. | en_US |
dc.description.sponsorship | Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. | en_US |
dc.language.iso | eng | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Fresas | en_US |
dc.subject.other | Strawberry | en_US |
dc.subject.other | Genome Editing | en_US |
dc.subject.other | CRISPR/Cas9 | en_US |
dc.subject.other | Flower | en_US |
dc.subject.other | Stamen | en_US |
dc.subject.other | TM6 | en_US |
dc.subject.other | AP3 | en_US |
dc.subject.other | Homeotic gene | en_US |
dc.title | Functional analysis of TM6 MADS-Box gene in the octoploid strawberry by CRISPR/Cas9 directed mutagenesis | en_US |
dc.type | info:eu-repo/semantics/conferenceObject | en_US |
dc.centro | Facultad de Ciencias | en_US |
dc.relation.eventtitle | Plant & Animal Genome XXVII | en_US |
dc.relation.eventplace | San Diego, EEUU | en_US |
dc.relation.eventdate | Enero 2019 | en_US |
dc.rights.cc | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |