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    •   RIUMA Principal
    • Investigación
    • Microbiología - (MIC)
    • MIC - Contribuciones a congresos científicos
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    Monitoring MBC-resistant isolates of the cucurbit powdery mildew, Podosphaera xanthii, using loop-mediated isothermal amplification (LAMP)

    • Autor
      Vielba-Fernández, AlejandraAutoridad Universidad de Málaga; De-Vicente-Moreno, AntonioAutoridad Universidad de Málaga; Perez-Garcia, AlejandroAutoridad Universidad de Málaga; Fernández-Ortuño, Dolores
    • Fecha
      2019-04-23
    • Palabras clave
      Fungicidas
    • Resumen
      Powdery mildew, caused by the fungus Podosphaera xanthii, is one of the most economically important diseases affecting cucurbit crops in Spain. Currently, chemical control is the most efficient management of the disease; however, P. xanthii isolates resistant to multiple classes of site-specific fungicides have been reported in the Spanish cucurbit powdery mildew population. In previous studies, resistance to the Methyl Benzimidazole Carbamates (MBCs) fungicides was found to be caused by the amino acid substitution E198A on β-tubulin. To detect MBC-resistant isolates in a faster, efficient and specific way than the traditional methods used to date, a loop-mediated isothermal amplification (LAMP) system was developed. Three sets of LAMP primers were designed and one set was optimize specifically to distinguish the E198A mutant genotype. The amplification products were visualized using gel electrophoresis and hydroxynaphtol blue (HNB), an azo dye that turn from violet to sky blue if the results were negative (E198) and positive (A198), respectively. Our results have proven that the LAMP technique is a specific and reproducible method that could be used to monitoring MBC-resistance of P. xanthii in the field.
    • URI
      https://hdl.handle.net/10630/17543
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    congreso Alemania.pdf (299.8Kb)
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    • MIC - Contribuciones a congresos científicos

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    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA